Author/Editor		Edhemović, Ibrahim
Title		Aktivnost fosfolipaze A2 v tumorjih širokega črevesa in danke
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		2002
Volume		str. 27
Language		slo
Abstract		Background: Type II phospholipase A2 (PLA2) is an enzyme important not only in the process of malignant transformation of the cells but also in the process of invasion. The aim of the present study was to investigate the expression of type II PLA2 in the carcinomas of the colorectum, peritumoral mucosa and in the mucosa distant from the tumor. Methods: Resection specimens from 57 patients with colorectal carcinoma (caecum 10, ascending colon 10, transverse colon 10, sigmoid colon 9, and rectum 18) were analysed immunohistochemically. Histological slides from paraffin blocks were stained by the human monoclonal type II PLA2 antibody ("Upstate Biotechnology", Lake Placid, NY 12946, USA. Antibody Class: IgGlk. Imunogen: HPC purifed human sperm phospholipase A2 - 14 kDa enzyme) using the standard DAKO peroxidase labelled Streptavidin-Biotin method and TechMate 500 stainer. Type II PLA2 expression was evaluated semiquantitatively according to the extensivity and intensivity of staining. For statistical evaluation the Kruskal-Wallis one way analysis of variance on ranks and the Mann-Whitney rank sum tests were used. Results: The highest expression of type II PLA2 was found in the peritumoral mucosa (median 4.00). It was much lower in the mucosa distant from the tumor (median 0.70) and almost absent in the tumor itself (median 0.00). All differences were statistically significant (Kruskal-Wallis test, all pairwise multiple comparison procedures - Dunn's Method: p<0.05). The expression of type II PLA2 was higher in the left colon and rectum than in the right colon (Mann-Whitney rank sum test: p<0.0ž). Conclusions: Our results suggest that there is variation of the type II PLA2 expression throughout the mucosa and tumors of the colorectum, which might reflect the progression of neoplastic process.
Descriptors		PHOSPHOLIPASES A COLORECTAL NEOPLASMS IMMUNOHISTOCHEMISTRY ANTIBODIES, MONOCLONAL