Author/Editor		Kataranovski, M; Milojević, G; Kandolf, L; Milosević, V
Title		Ski-organ culture as an approach in evaluation of nitric oxide (NO) involvement in contact hypersensitivity expression
Type		članek
Source		Acta Dermatovenerol Alp Pannon Adriat
Vol. and No.		Letnik 11, št. 1
Publication year		2002
Volume		str. 3-10
Language		eng
Abstract		Objective. Contact hypersensitivity (CHS) is a local inflammatory response of the skin following challenge of hapten-sensitized animals. The intensity of inflammation could be quantified by ear swelling, a classical manifestation of contact hypersensitivity. In this study, skin-organ culture system was employed to evaluate involvement of nitric oxide (NO) in the expression of CHS in rats. Methods. Nitrite accumulation in conditioned medium (CM) of rat ear skin following challenge with DNCB was determined by Griess assay. The experiment was conducted on two genetically different rat strains (AO and DA), which differ in the magnitude of ear swelling response. Results. Dose-dependent increase in nitrite levels was noted in CM following application of 0.65% and 1.3% DNCB to the ears of sensitized rats of both strains. The response was higher in DA rats, displaying a more vigorous ear swelling. Correlation between ear swelling and the levels of nitrites in CM of both strains was found. Decrease in nitrite levels was noted in CM in the presence of aminoguanidine, a known inhibitor with marked specificity for the inducible isoform of the NO synthase in rodents. Conclusion. Presented data demonstrated the utility of skin organ-culture system in detection of NO production during the expression of CHS.
Descriptors		DERMATITIS, ALLERGIC CONTACT NITRIC OXIDE NITRITES DINITROCHLOROBENZENE RATS CELLS, CULTURED DOSE-RESPONSE RELATIONSHIP, DRUG