Author/Editor     Manček, Mateja; Pristovšek, Primož; Jerala, Roman
Title     Identification of LPS-binding peptide fragment of MD-2, a toll-receptor accessory protein
Type     članek
Source     Biochem Biophys Res Commun
Vol. and No.     Letnik 292, št. 4
Publication year     2002
Volume     str. 880-5
Language     eng
Abstract     Members of the toll-like receptor family are crucial in recognition of microbial pathogens as part of innate immune response. MD-2, an accessory protein to TLR4, present on the extracellular side of the membrane is needed to initiate the signal transduction. We have identified a 15 amino acid region of human MD-2 that contains several features of other lipopolysaccharide (LPS) binding proteins and peptides. In vitro LPS neutralization by this peptide was observed and confirmed by 2D transferred NOESY NMR experiments. NMR experiments have also shown binding of the MD-2 peptide to lipoteichoic acid (LTA) but not to peptidoglycan. Furthermore this peptide inhibited growth of gram-negative and to a lower extent of some gram-positive bacteria. Our results indicate that this region of MD-2 might be responsible for binding of LPS and confirms the role of MD-2 as an accessory protein in LPS signaling bestowing the Toll receptors their specificity.
Descriptors     PEPTIDE FRAGMENTS
LIPOPOLYSACCHARIDES
ANTIGENS, SURFACE
TEICHOIC ACIDS
SIGNAL TRANSDUCTION
RECEPTORS, CELL SURFACE
PROTEIN BINDING
PEPTIDOGLYCAN
MICROBIAL SENSITIVITY TESTS
MEMBRANE GLYCOPROTEINS
NUCLEAR MAGNETIC RESONANCE
IMMUNITY, NATURAL
BINDING SITES
GRAM-NEGATIVE BACTERIA
GRAM-POSITIVE BACTERIA