Author/Editor		Benčina, M; Legiša, M
Title		Stress mediated changes in expression of the pkaC gene, encoding the catalytic subunit of cAMP-dependent protein kinase, in Aspergillus niger
Type		članek
Source		Pflugers Arch
Vol. and No.		Letnik 439, št. 3 Suppl
Publication year		2000
Volume		str. R76-8
Language		eng
Abstract		The transcriptional regulation of the pkaC gene, encoding the catalytic subunit of cAMP-dependent protein kinase from Aspergillus niger, was analysed under different environmental conditions. Quantitative determination of pkaC transcript showed a significant decrease in concentration of specific mRNA immediately after a temperature, hypoosmotic and hyperosmotic shock followed by stimulated synthesis. The amount of pkaC mRNA as well as PKA enzymatic activity steadily decreased during the initial phase of growth in 15% sucrose medium while a slight increase was observed at the time of a change in morphology from bulbous cells to filamentous growth. Transcriptional alternation might be mediated by multiple putative stress elements in the promoter region of pkaC gene.
Descriptors		STRESS GENES, FUNGAL GENE EXPRESSION CYCLIC AMP-DEPENDENT PROTEIN KINASES ASPERGILLUS NIGER TEMPERATURE POLYMERASE CHAIN REACTION OSMOTIC PRESSURE FERMENTATION