Author/Editor		Pflegerl, K; Podgornik, A; Berger, E; Jungbauer, A
Title		Direct synthesis of peptides on convective interaction media monolithic columns for affinity chromatography
Type		članek
Source		J Com Chem
Vol. and No.		Letnik 4, št. 1
Publication year		2002
Volume		str. 33-7
Language		eng
Abstract		Solid-phase peptide synthesis was performed on glycidyle methacrylate-co-ethylene dimethacrylate monoliths using Fmoc chemistry. The native epoxy groups were amino-functionalized by reaction with ethylenediamine or ammonia ions. A peptide directed against human blood coagulation factor VIII was synthesized as a model peptide. Amino acid analysis revealed the correct amino acid ratio as present in the sequence. The ligand density of 5 micromol/mL was equal to that achieved with conventional peptide immobilization via epoxy groups. These supports were directly used as peptide affinity chromatography matrixes. The functionality of the CIM monolithic supports was proven by affinity chromatography of factor VIII. The ammonia-functionalized support performed with low hydrophobicity and did not show unspecific adsorption of proteins.
Descriptors		PEPTIDES CHROMATOGRAPHY, AFFINITY METHACRYLATES EPOXY COMPOUNDS ETHYLENEDIAMINES FACTOR VIII AMINO ACID SEQUENCE LIGANDS