Author/Editor		Marin, J; Jeler-Kačar, D; Levstek, V; Maček, V
Title		Persistence of viruses in upper respiratory tract of children with asthma
Type		članek
Source		J Infect
Vol. and No.		Letnik 41, št. 1
Publication year		2000
Volume		str. 69-72
Language		eng
Abstract		Objectives: Nasopharyngeal swabs of 50 asthmatic children in the symptom-free period were examined for the presence of adenoviruses, rhinoviruses and coronaviruses. A control group of 20 healthy individuals was included in this study. Methods: A polymerase chain reaction was used to detect adenovirus DNA and rhinovirus and coronavirus complementary DNA. The fragments of amplified genetic material were visualized with the use of agarose gel electrophoresis. Results: Adenovirus DNA was found in 78.4% of asthmatic children, rhinovirus RNA in 32.4% and coronavirus RNA in 2.7%. Adenovirus DNA was detected in one of the 20 nasopharyngeal swabs of healthy controls; the rest of the control samples were negative. Conclusions: The persistent presence of viruses in the upper respiratory tract of asthmatic children shows a possible connection between viral infections and asthma.
Descriptors		RESPIRATORY TRACT INFECTIONS NASOPHARYNGEAL DISEASES ASTHMA CHILD ADULT RHINOVIRUS POLYMERASE CHAIN REACTION PICORNAVIRIDAE INFECTIONS NASOPHARYNX ADENOVIRIDAE ADENOVIRIDAE INFECTIONS CORONAVIRIDAE INFECTIONS CORONAVIRUS DNA PRIMERS DNA, VIRAL ELECTROPHORESIS, AGAR GEL