Author/Editor		Hahn, R; Podgornik, A; Merhar, M; Schallaun, E; Jungbauer, A
Title		Affinity monoliths generated by in situ polymerization of the ligand
Type		članek
Source		Anal Chem
Vol. and No.		Letnik 73
Publication year		2001
Volume		str. 5126-32
Language		eng
Abstract		An affinity monolith with a novel immobilization strategy was developed leading to a tailored pore structure. Hereby the ligand is conjugated to one of the monomers of the polymerization mixture prior to polymerization. After the polymerization, a monolithic structure was obtained either ready to use for affinity chromatography or ready for coupling of additional ligand to further increase the binding capacity. The model ligand, a peptide directed against lysozyme, was conjugated to glycidyl methacrylate prior to the polymerization. With this conjugate, glycidyl methacrylate, and ethylene dimethacrylate, a monolith was formed and tested with lysozyme. A better ligand presentation was achieved indicated by the higher affinity constant compared to a conventional sorbent.
Descriptors		CHROMATOGRAPHY, AFFINITY METHACRYLATES MURAMIDASE POLYMERS CROSS-LINKING REAGENTS EPOXY COMPOUNDS LIGANDS PEPTIDES