Author/Editor		Mihelič, Igor; Koloini, Tine; Podgornik, Aleš; Štrancar, Aleš
Title		Dynamic capacity studies of CIM (convective interaction media) monolithic columns
Type		članek
Source		J High Resolut Chromatogr
Vol. and No.		Letnik 23, št. 1
Publication year		2000
Volume		str. 39-43
Language		eng
Abstract		The characterization of CIM DEAE monolithic columns in terms of dynamic binding capacity is presented in this paper. Breakthrough experiments were performed for capacity determination. Bovine serum albumin (BSA) was used as a model protein. It is shown that CIM monolithic columns have good batch-to-batch reproducibility as well as long-term stability. The experiments performed under different linear velocities demonstrated that the dynamic capacity is unaffected at least up to a linear velocity of 2450 cm/h. Furthermore, the breakthrough curve slope is constant, indicating that the capacity would remain constant at even higher linear velocities. The adsorption isotherm of BSA dissolved in 20 mM Tris-HCl buffer shows a constant capacity of around 30 mg/mL of support down to a concentration of 20 pg/mL. The capacity is substantially influenced by the ionic strength; however, 20% of the maximal capacity is still preserved at 0.3 M NaCI.
Descriptors		SERUM ALBUMIN, BOVINE TROMETHAMINE CHROMATOGRAPHY, DEAE-CELLULOSE