Author/Editor     Costa, Silvano; Terzano, Patrizia; Bovicelli, Alessandro; Martoni, Andrea; Angelelli, Bruna; Santini, Donatella; Ceccarelli, Claudio; Lipponen, Pertti; Eržen, Mojca; Syrjaenen, Stina
Title     CD44 isoform 6 (CD44v6) is a prognostic indicator of the response to neoadjuvant chemotherapy in cervical carcinoma
Type     članek
Source     Gynecol Oncol
Vol. and No.     Letnik 80, št. 1
Publication year     2001
Volume     str. 67-73
Language     eng
Abstract     Objective: Theclinical efficacy of neoadjuvant chemotherapy (NAC) in distinct groups of cervical cancer patients has been well documented, but parameters at the cellular level regulating the different responsiveness to this treatment have not been adequately explored. Method: A series of 21 patients with stage Ib and IIa bulky cervical carcinomas were treated by preoperative NAC with three courses of cisplatin, epirubicin, etoposide, and bleomycin prior to radical hysterectomy, and subsequently followed up for a mean of 52.3 months. Biopsies taken prior to NAC and operative specimens were subjected to immunohistochemical (IHC) staining for alpha-catenin, beta-catenin, E-cadherin, and CD44 isoform 6 (CD44v6), to uncover the role of adhesion molecules as determinants of the response to NAC and disease outcome. Results: Seven of the twenty-one (33.3%) women died of the disease; adenosquamous (n = 4 cases) histology (RR 4.50, 95% CI 1.85-10.68) and lymph node involvement (RR 6.00, 95% CI 0.42-85.26) were significant determinants of nonsurvival. All 21 carcinomas were human papillomavirus DNA positive. The factors predicting the response to NAC in univariate analysis were: CD44v6 expression in the pre-NAC and post-NAC samples (P = 0.00056 and P = 0.00336, respectively). In multiple logistic regression analysis, the factors with independent predictive value for response to NAC were CD44v6 expression prior to (P = 0.0099) and after (P = 0.0470) NAC. In univariate survival analysis, the most significant (P < 0. 001) predictors of recurrence-free survival (RFS) were age and number of lymph nodes removed. In multivariate survival analysis, the independent predictor for RFS was only histological type (P = 0. 0064). Overall survival (OS) was predicted in a Cox model by recurrence (P = 0.0033), CD44v6 expression after NAC (P = 0.013), and patient's age (P = 0.039). (Abstract truncated at 2000 characters)
Descriptors     ANTIGENS, CD44
ANTINEOPLASTIC AGENTS, COMBINED
CERVIX NEOPLASMS
GLYCOPROTEINS
TUMOR MARKERS, BIOLOGICAL
ADENOCARCINOMA
AGE FACTORS
BLEOMYCIN
CADHERINS
CARCINOMA, ADENOSQUAMOUS
CISPLATIN
CYTOSKELETAL PROTEINS
DISEASE-FREE SURVIVAL
EPIRUBICIN
ETOPOSIDE
HYSTERECTOMY
IMMUNOHISTOCHEMISTRY
SURVIVAL ANALYSIS