Author/Editor		Cencič, Avrelija; Hennry, Celine; Lefevre, Francois; Huet, Jean Claude; Koren, Srečko; La Bonnardiere, Claude
Title		The porcine trophoblastic interferon-gamma, secreted by a polarized epithelium, has specific structural and biochemical properties
Type		članek
Source		Eur J Biochem
Vol. and No.		Letnik 269, št. 11
Publication year		2002
Volume		str. 2772-81
Language		eng
Abstract		At the time of implantation in the maternal uterus, the trophectoderm of the pig blastocyst is the source of a massive secretion of interferon-gamma (IFN-gamma), together with lesser amounts of IFN-delta, a unique species of type I IFN. This trophoblastic IFN-gamma (TrIFN-gamma) is an unprecedented example of IFN-gamma being produced spontaneously by an epithelium. We therefore studied some of its structural and biochemical properties, by comparison with pig IFN-gamma from other sources, either natural LeIFN-gamma (from adult leucocytes), or recombinant. Biologically active TrIFN-gamma is a dimeric molecule, of which monomers are mainly composed of a truncated polypeptide chain with two glycotypes, unlike LeIFN-gamma which is formed of at least two polypeptide chains and four glycotypes. TrIFN-gamma collected in the uterus lumen was enzymatically deglycosylated and analysed by mass spectrometry (MALDI-TOF). The data revealed that the more abundant polypeptide has a mass of 14.74 kDa, corresponding to a C-terminal cleavage of 17 residues from the expected 143-residue long mature sequence. A minor polypeptide, with a mass of 12.63 kDa, corresponds to a C-terminal truncation of 36 amino acids. MALDI-TOF analysis of tryptic peptides from the glycosylated molecule(s) identifies a single branched carbohydrate motif, with six N-acetylgalactosamines, and no sialic acid. The only glycan microheterogeneity seems to reside in the number of l-fucose residues (one to three). The lack of the C-terminal cluster of basic residues, and the presence of nonsialylated glycans, result in a very low net charge of TrIFN-gamma molecule. However, the 17-residue truncation does not affect the antiproliferative activity of TrIFN-gamma on different cells, among which is a porcine uterine epithelial cell line. (Abstract truncated at 2000 characters).
Descriptors		INTERFERON TYPE II TROPHOBLAST CELL POLARITY EPITHELIUM LEUKOCYTES SWINE