Author/Editor		Gašperšič, R; Štiblar-Martinčič, D; Skalerič, U
Title		Vpliv podkožnega dovajanja TNF-alfa na razvoj parodontitisa pri podganah
Translated title		Influence of subcutaneous administration of TNF-alpha on experimental periodontitis in rats
Type		članek
Source		Zobozdrav Vestn
Vol. and No.		Letnik 57, št. 3-4
Publication year		2002
Volume		str. 112-6
Language		slo
Abstract		A number of studies have shown that inflamed periodontal tissues contain TNF-alpha, a proinflammatory cytokine which plays an important role in the development of periodontal disease. In this study, conducted on rats, the influence of recombinant human TNF-alpha (rhTNF-a) on inflammatory reaction and periodontal breakdown was analyzed in a model of experimental periodontitis, induced by placing silk ligatures around the maxillary right second molar. Ten rats were divided into two groups with five animals in each. The first group received rhTNF-alpha, applied subcutaneously via osmotic pumps over a period of 2 wk; in the second group, PBS was administered in the same manner. Inflammatory reaction and degradation of periodontal tissues were evaluated morphometrically on HE stained sections. When used alone, ligation resulted in accelerated periodontal degradation, while rhTNF-alpha had no significant effect. The combined use of rhTNF-alpha and ligation resulted in a significantly greater inflammatory reaction and periodontal breakdown than either treatment alone. We conclude that subcutaneous administration of rhTNF-alpha accelerates the progression of experimental periodontitis in rats.
Descriptors		PERIODONTITIS TUMOR NECROSIS FACTOR INJECTIONS, SUBCUTANEOUS RATS, WISTAR BIOMETRY INFLAMMATION