| Author/Editor | Starčič, Marjanca; Johnson, James R; Stell, Adam L; van der Goot, Jeanet; Hendriks, Henno GCJM; van Vorstenbosch, Camillo; van Dijk, Linda; Gaastra, Wim | |
| Title | Haemolytic Escherichia coli isolated from dogs with diarrhea have characteristics of both uropathogenic and necrotoxigenic strains | |
| Type | članek | |
| Source | Vet Microbiol | |
| Vol. and No. | Letnik 85 | |
| Publication year | 2002 | |
| Volume | str. 361-77 | |
| Language | eng | |
| Abstract | Twenty-four haemolytic Escherichia coli strains were isolated from dogs with diarrhea. The strains were serotyped and analysed by polymerase chain reaction (PCR) for genes encoding virulence factors associated with E. coli that cause diarrhea in animals. Adhesion antigen production was deduced from haemagglutination experiments. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of heat extracts was also used as an indication for the production of adhesive structures. The majority of the strains was shown to produce this type of virulence factor. Adhesion and invasion tests of the strains and Caco-2 cells showed that all strains adhered and that two were invasive. The two invasive strains were positive in the intimin PCR and one of them also contained genes encoding CS31A. The PCR for heat stable toxin (ST) was positive in only four strains, as was the presence of F17 fimbrial genes. Surprisingly, 19 strains had intact P fimbrial operons, coding for an adhesin involved in urinary tract infection (UTI). The cytotoxic necrotising factor 1 (CNFI) gene, also mainly found in UTI was likewise detected in these 19 strains. Cytolethal distending toxin (Cdt) genes were found in five strains. The high number of strains positive for CNFl and P fimbriae prompted us to test the strains in a multiplex PCR used to test E. coli isolated from UTI in various species for 30 virulence associated genes. The data showed that the majority of the diarrhea | |
| Descriptors | BACTERIAL ADHESION DIARRHEA ESCHERICHIA COLI ESCHERICHIA COLI INFECTIONS ADHESINS, BACTERIAL BLOTTING, SOUTHERN BLOTTING, WESTERN CACO-2 CELLS MICROSCOPY, ELECTRON POLYMERASE CHAIN REACTION SEQUENCE ANALYSIS, DNA VIRULENCE |