| Author/Editor | Meglič, Vladimir; Virant-Doberlet, Meta; Šuštar-Vozlič, Jelka; Čokl, Andrej | |
| Title | Isozyme variation in the southern green stink bug Nezara viridula (L.) (Heteroptera: Pentatomidae) | |
| Translated title | Raznovrstnost izoencimov pri stenici vrste Nezara viridula (L.) (Heteroptera: Pentatomidae) | |
| Type | članek | |
| Source | Acta Entomol Slov | |
| Vol. and No. | Letnik 10, št. 1 | |
| Publication year | 2002 | |
| Volume | str. 55-64 | |
| Language | eng | |
| Abstract | The southern green stink bug Nezara viridula (Heteroptera: Pentatomidae) is a cosmopolitan species with a still uncertain geographical origin. The ancestral home of this species is supposed to be Africa and/or the Mediterranean; presumably it was spread world-wide during the last two centuries by human trade and agriculture. Bugs found today on different continents do not differ morphologically, however there are substantial differences in their mating behaviour. We used horizontal starch gel electrophoresis to determine the suitability of biochemical markers for assessment of genetic variation between geographically isolated populations of N. viridula. The initial survey of populations from Slovenia, France, French West Indies, and Brazil resulted in the resolution of polymorphic banding patterns within the following enzyme systems: glucosephosphate isomerase (GPI), isocitrate dehydrogenase (IDH) malate dehydrogenase (MDH), malic enzyme (ME), mannosephosphate isomerase (MPI) and phosphoglucomutase (PGM). Results indicate that in some enzyme systems such as PGM and MPI there are consistent differences among the tested populations. | |
| Summary | Stenica vrste Nezara viridula (Heteroptera: Pentatomidae) je kozmopolitska vrsta, katere geografski izvor je še vedno nejasen. Verjetno izvira iz Afrike in/ali sredozemske regije in se je s trgovanjem in širjenjem kmetijstva v zadnjih 250 letih razširila po vsem svetu. Stenice te vrste, ki jih najdemo na različnih celinah, se med seboj morfološko ne razlikujejo, vendar obstajajo v paritvenem vedenju stenic iz geografsko ločenih populacij očitne razlike. S pomočjo vodoravne škrobne elektroforeze smo želeli preveriti uporabnost biokemičnih markerjev za določanje genske raznolikosti geografsko ločenih populacij stenice vrste N. viridula. Testirali smo populacije iz Slovenije, Francije, Zahodne Indije (Guadeloupe) in Brazilije. Domnevne polimorfne alele smo našli pri naslednjih encimskih sistemih: glukozefosfatizomeraza (GPI), izocitratdehidrogenaza (IDH) malatdehidrogenaza (MDH), malični encim (ME), manozefosfatizomeraza (MPI) in fosfoglukomutaza (PGM). Rezultati kažejo, da za nekatere encimske sisteme (PGM in MPI) obstajajo med populacijami značilne razlike. | |
| Descriptors | HEMIPTERA ISOENZYMES MUSCLE, SKELETAL ALLELES GLUCOSEPHOSPHATE DEHYDROGENASE ISOCITRATE DEHYDROGENASE MALATE DEHYDROGENASE MANNOSEPHOSPHATE ISOMERASE ELECTROPHORESIS, STARCH GEL |