Author/Editor		Skvarč, Miha; Ećimović, Katarina Kaja
Title		Primerjava občutljivosti bakterije Helicobacter pylori za antibiotike z agar dilucijsko metodo in E-testom
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		2001
Volume		str. 38
Language		slo
Abstract		BACKGROUND. Helicobacter pylori is a gram-negative, spiral-shaped bacterium, primarily found in the gastric mucosa. The first isolation from the human stomach was made in 1982 in Australia. It is estimated that over 50% of the population in the developed countries is infected. The infection with H. pylori is associated with the peptic ulcer disease, gastric adenocarcinoma and MALT lymphoma. The treatment with antimicrobial agents successfully eradicates H. pylori from the gastric mucosa, producing favorable clinical response especially for the patients with the peptic ulcer disease. The resistance against antibiotics in particular the metronidazole and the clarithromycin is rising and determents clinical outcome of the antibiotic treatment. AIM. The determination of the in vitro susceptibility of H. pylori to antimicrobial agents is of vital importance for the successful treatment of infection The susceptibility testing is mainly performed with the E-test. The reference method is agar dilution method. The aim of this study is to compare the results obtained by the E-test and agar dilution method. HYPOTHESIS. We compared the results obtained by the E-test with results got with the agar dilution method. We claim that the two methods for determination of the susceptibility of H. pylori to antibiotics are equally reliable. METHODS. 41 H. pylori strains isolated from patients were tested against 6 antibiotics with the agar dilution method. The E-test results and strains were obtained from the previous study. The susceptibility testing was carried out on Mueller-Hinton agar containing 5% of sheep blood with incorporated antibiotics. The concentration range was from 0.004 to 256 micro g/ml. We also tested the ATCC 43504 control strain with the known MIC values. The results got with two methods were statistically compared with paired t test. (Abstract truncated at 2000 characters).
Descriptors		HELICOBACTER PYLORI MICROBIAL SENSITIVITY TESTS COLONY COUNT, MICROBIAL ANTIBIOTICS AMOXICILLIN CLARITHROMYCIN ROXITHROMYCIN AZITHROMYCIN CIPROFLOXACIN METRONIDAZOLE