Author/Editor		Zavašnik-Bergant, Valentina; Kopitar-Jerala, Nataša; Bevec, Tadeja; Kos, Janko
Title		Characterization of monoclonal antibodies against MHC class II-associated p41 invariant chain fragment
Type		članek
Source		Radiol Oncol
Vol. and No.		Letnik 36, št. 2
Publication year		2002
Volume		str. 185-8
Language		eng
Abstract		Mouse monoclonal antibodies directed against human MHC class 11-associated p41 invariant chain fragment have been generated. Mice were immunized with human recombinant li-isoform p26: For hybridoma production mouse splenocytes and myeloma cells were fused. Hybridoma cells were screened using ELISA and immunoblotting. Three cell lines (42B10, 42G11 and 43C8) were used for production of specific antibodies, which reacted with p41 fragment and did not bind to cathepsins L or S or their proenyzmes: As primary antibody for immunofluorescence staining of lymph node tissue sections clone 2C12 MAb was selected. Specific localization of p41 fragment in certain cells in lymph nodes was observed.
Descriptors		MAJOR HISTOCOMPATIBILITY COMPLEX ANTIBODIES, MONOCLONAL HYBRIDOMAS CATHEPSINS