Author/Editor		Simpson, DJ; Fryer, AA; Grossman, AB; Wass, JAH; Pfeifer, M; Kros, JM; Clayton, RN; Farrell, WE
Title		Cyclin D1 (CCND1) genotype is associated with tumour grade in sporadic pituitary adenomas
Type		članek
Source		Carcinogenesis
Vol. and No.		Letnik 22, št. 11
Publication year		2001
Volume		str. 1801-7
Language		eng
Abstract		The cyclin D1 (CCNDI ) gene contains a frequent A/G polymorphism within the splice donor region of exon 4/intron 4. CCNDI genotype is associated with clinical outcome in a number of malignancies although prognostic significance varies with tumour type. We examined CCNDI allele frequencies and genotype distribution in 294 patients with sporadic pituitary adenomas of various histologies. CCNDI allele frequencies and distribution of genotypes were similar in the 294 cases compared with previously reported control populations. Analysis according to tumour subtype showed no statistical difference in allele frequencies compared with controls. However, CCNDl genotype distribution in the somatotrophinomas showed a significant difference compared with normal controls (P = 0.008). We next examined CCNDl allele frequencies and genotype distribution across the tumour grades. Within the total tumour cohort the CCNDl allele frequencies showed a significant inverse relationship across the tumour grades (P = 0.005). The CCNDI A allele progressively increased from grade 1 (0.37) through to grade 4 (0.62) tumours, whilst the CCNDl G allele frequency progressively decreased from grade 1 (0.63) through to grade 4 (0.38) tumours. Trend analysis of CCNDI genotypes showed a significant progressive increase in AA frequency from grade 1 (15 % ) through to grade 4 (46 %) tumours (P = 0.005). The CCNDI GG genotype progressively decreased from grade 1 (41 % ) through to grade 4 (23 % ) tumours (P = 0.204). No statistical significance was observed between CCNDI AG genotype and tumour grades. While the functional significance of the observed segregation of the CCNDI A/G polymorphism and tumour grade is unclear, our data suggest that CCNDI allele frequencies and genotype distributions show significant differences between tumour grades in sporadic pituitary adenomas. (Abstract truncated at 2000 characters).
Descriptors		ADENOMA CYCLINS PITUITARY NEOPLASMS CODON PROLACTINOMA CORTICOTROPIN DNA PRIMERS EXONS GENOTYPE INTRONS NEOPLASM STAGING POLYMERASE CHAIN REACTION POLYMORPHISM (GENETICS) RECURRENCE RETROSPECTIVE STUDIES SEQUENCE ANALYSIS, DNA SOMATOTROPIN