Author/Editor		Eržen, Ida; Sketelj, Janez; Agbulut, Onnik; Angaut-Petit, Denise; Butler-Browne, Gillian S
Title		Coexpression of myosin heavy chain 2b with myosin heavy chain 1 - fact or artefact?
Type		članek
Source		Basic Appl Myol
Vol. and No.		Letnik 11, št. 3
Publication year		2001
Volume		str. 121-7
Language		eng
Abstract		In skeletal muscle, pure fibres expressing one myosin heavy chain (MyHC) isoform, and intermediate fibres, expressing two and exceptionally three MyHCs have been described. When skeletal muscle adapts its fibre type profile to changed functional demands MyHC isoform transformation follows the pathway: MyHC-1 - MyHC-2a - MyHC-2x/d - MyHC-2b. Therefore, in hybrid fibres only successive isoforms from the proposed pathway should coexist. However, jump fibres in which MyHC-1 is co-expressed with MyHC-2x/d have been described recently. The present study describes possible coexpression of MyHCI with MyHC-2b in transforming as well as in normal control mouse and rat muscle fibres. The study is only descriptive and provides not sufficient proof to exclude the possible artefact resulting from unknown technical reasons.
Descriptors		MUSCLE, SKELETAL MYOSIN HEAVY CHAINS MICE RATS, WISTAR IMMUNOHISTOCHEMISTRY ELECTROPHORESIS BLOTTING, WESTERN