Author/Editor		Leštan, Domen; Černilec, Maja; Perdih, Anton
Title		Determination of ligninase activity in P. chrysosporium pellets with diffuse reflectance spectrophotometry
Type		članek
Source		Appl Microbiol Biotechnol
Vol. and No.		Letnik 38
Publication year		1993
Volume		str. 570-3
Language		eng
Abstract		Diffuse reflectance spectrophotometry was applied for measuring ligninase activity in pellets of Phanerochaete chrysosporium. Enhanced ligninase activity in pellets and in growth medium were detected in cultures supplemented with oleic acid emulsified with Tween 80, Tween 80, hydrophilic (alcoholic) residue of Tween 80 hydrolysate, Tween 20, and (150E)C18:1. In cultures with low extracellular ligninase activity, low activity in pellets was also observed. Our results indicate that the stimulatory effect of the tested surfactants cannot be contributed solely to promotion of ligninases through the cell membrane.
Descriptors		BASIDIOMYCETES LIGNIN FERMENTATION SPECTROPHOTOMETRY