Author/Editor     Vlaisavljević, Veljko; Reljič, Milan
Title     Does the in vitro fertilization procedure require bacteriological investigation of normozoospermic ejaculate?
Type     članek
Source     Gynaecol Perinatol
Vol. and No.     Letnik 11, št. 2
Publication year     2002
Volume     str. 69-72
Language     eng
Abstract     The aim of our study was to compare the fertilization rate in IVF procedures in cases where the ejaculate was not subjected to bacteriologic investigation and in those where bacteriologic investigation of the ejaculate was performed and after which antibiotic therapy followed. Material From among 241 normozoospermiacs included in the IVF procedure, 56 cases were self-selected for bacteriologic investigation of ejaculate. The fertilization rate and the percentage of cycles with total fertilization failure following IVF was compared with the remaining 185 IVF cycles before which no bacteriologic investigation of ejaculate had been done and the men have received no antibiotic therapy. Results. Bacteria in the ejaculate were isolated in 95% (53/56). The culture remained sterile in 5% (3/56). The most frequently isolated bacteria were Peptostreptococci in 26%, Streptococcus viridans in 24%, Enterococci in 22%, alpha hemolytic Streptococci in 20%, anaerobic gram-positive bacilli in 18%, anaerobic gram-negative bacilli in 13% and Diphtheroids in 10%. From among the less frequent bacteria, Staphylococcus epidermis and E. coli were assessed in 4%. In 22% of samples, only one bacterial species was isolated, in 38% two and in 40% three or more. There was no statistically significant difference between the groups with regard to the number of oocytes and embryos, the fertilization rate and the percentage of cycles without fertilization. Conclusion. According to our experience, bacteriologic investigation of normozoospermic ejaculate in the IVF procedure and subsequent antibiotic treatment of asymptomatic men with bacteria in the ejaculate bears no influence on the fertilization rate and pregnancy rate in IVF procedure.
Descriptors     FERTILIZATION IN VITRO
SEMEN
BACTERIA
ANTIBIOTICS