Author/Editor		Pretnar, Gorazd; Steindl, Franz
Title		Time dependent cell growth in biomedical research with general cell screening system
Type		članek
Source		In: Kokol P, Stiglic B, Zorman M, et al, editors. Proceedings of the 15th IEEE symposium on computer-based medical systems (CBMS 2002); 2002 Jun 4-7; Maribor. Los Alamitos: Institute of electrical and electronics engineers,
Publication year		2002
Volume		str. 41-6
Language		eng
Abstract		Modern and effective biomedical research needs new and effective methods. Use of 96 wellmicrotter lates allows the performance of many experiments and samples under the same conditions. Methods to determine cell numbers in 96 well-microtiter plates are all endpoint methods. Methods for determination of cell numbers such as measuring the incorporation of radioactive nucleides like thymidine, MTT, XTT, MTS, lactate dehydrogenase, acid phosphatase, etc. allow only one determination per plate and the cells are lost. Usually such assays are performed according to concentration dependance. Other parameters and cell growth dynamics remained unrevealed. To reveal cell growth dynamics multiple assays should be performed. Such experiment approach would be expencive and time consuming so it is rarely performed. To fill this gap IAM and SLT Labinstruments developed GCSS (General Cell Screening System). GCSS is a powerful hardware and software system that enables continuous monitoring of the cell growth without any treatment or stain. The method is based on a high resolution turbiditžt. measurement (700 nm) per formed directly in the 96 well cell culture plate. The system consists of the GCSS-reader and 8 channel photometer, (the beam has the form of a narrow rectangle), the GCSS- plate with a new form of the wells, an Apple Macintosh computer and the GCSSSoftware. We chose classic bone marrow colony count assay, which is tipical assay scored after seven days of incubation and based on one measurement only. GCSS allowed us, to seed bone marrow cells in microtiter plates in medium with different concentrations of haemopoietic growth factors and performed multiple measurements. In this experiment we observed bone marrow cell growth derived from interferon alpha treated mice and compare the cell growth from placebo treated mice. (Abstract truncated at 2000 characters)
Descriptors		CELL DIVISION BONE MARROW INTERFERON-ALPHA MICE, INBRED C57BL CELL COUNT DIAGNOSIS, COMPUTER-ASSISTED