| Author/Editor | Prelovšek, Petra-Maja | |
| Title | Histokemijska in ultrastrukturna analiza pigmentnih celic v jetrih močerila (Proteus anguinus, Amphibia: Urodela) | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Medicinska fakulteta | |
| Publication year | 2002 | |
| Volume | str. 129 | |
| Language | slo | |
| Abstract | Proteus anguinus a blind cave salamander is very well adapted to poor and discontinuous food supplies and can survive during long periods of food deprivation. Knowledge about structure of its liver will provide the morphological bases for further investigations of structural changes during food deprivation. Liver of P. anguinus contains numerous, large clusters of pigment cells (PC). PC in the livers of amphibians and reptiles are resident macrophages, also known as Kupffer cells. They contain various amounts of melanin, hemosiderin and lipofuscin. Liver tissue of P. anguinus was studied by histochemical methods in order to analyse the nature of pigments present in PC. Fine stntcture of these cells was analysed by transmission electron microscopy. Clusters of PC contain yellow-brown and dark brown pigments. Yellow-brown pigments are positive after Perls' reaction. Ultrastructural analysis confumed the presence of numerous siderosomes containing electron-dense iron containing particles. Dark brown pigments have histochemical and ultrastructural characteristics of melanin. Pigment cells in the liver of P. anguinus can be divided into 3 different types: (1) pigment cells with siderosomes (PC-S), (2) pigment cells with vacuoles (PC-V) and (3) pigment cells with melanosome complexes (PC-M).The prevailing types of pigment cells are PC-S. They contain numerous siderosomes and melanosomogenesis centres. Ultrastructural characteristics of melanosomes and positive DOPA-oxidase reaction confirm the melanosome production in these cells. Main characteristic of PC-V are numerous vacuoles. Most of the vacuoles appear empty; some contain membranous or particulate matter. Lipofuscin bodies are rare, and are present only in few PC-S and PC-V. PC-M appear to be less numerous than PC-S or PC-V. Their main inclusion bodies are melanosome complexes. (Abstract truncated at 2000 characters). | |
| Descriptors | LIVER PIGMENTS IRON MELANINS HEMOSIDERIN HISTOCYTOCHEMISTRY PROTEIDAE MICROSCOPY, ELECTRON, SCANNING |