Author/Editor		Pleskovič, Aleš; Rijavec, Boris
Title		Morfološke spremembe v koronarnih arterijah pri bolnikih okuženih z bakterijo Chlamydia pneumoniae
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		2001
Volume		str. 40
Language		slo
Abstract		INTRODUCTION. Diffuse and distal coronary artery disease (DDCD) is the most serious atherosclerotic process of coronary arteries characterised by long atherosclerotic segments involving all parts of the vessel wall. Surgical treatment is still very complicated and DDCD can be solved by endarterectomy procedure. Recently infective genesis has been proposed for DDCD. PURPOSE. In theresearch study pathomorphological changes in DDCD were investigated in patients who were serologically positive for Chlamidia pneumoniae (C. pneumoniae). HYPOTHESIS. - Chronic persistent infection with C. pneumoniae could be one of the cause for the development of the DDCD. - Serologically proved chronic persistent infection with C. pneumoniae involves specific pathological changes in coronary vessel wall. - C. pneumoniae could be detected in atherosclerotic parts of coronary arteries as well as in healthy segments. METHODS. In 10 male patients with diffuse and distal coronary artery disease and with positive serologic test of microimmunofluorescence (micro-IF) for C. pneumoniae morphological and immunohistological analyses of endaterectomy sequesters were performed. With polimerase chain reaction (PCR) method in 3 out of 10 patients, the DNA particles of C. pneumoniae were investigated in macroscopically normal and aterosclerotic parts of vessel wall. Histological sections were stained with HE, Masson trichrom and Weigert van Gieson staining. Fibroprofilerative changes, cellular infiltration of vessel wall, neoanginogenesis and pathology of small vessels were morphologically analysed. With immunohistochemical method limphocyte T, lymphocyte B, macrophages, plasma cells and endothelial cells were investigated. Proliferative activity of the cells in the wall of sequesters was estimated by MIB-1 antibody for Ki-67 antigene. (Abstract truncated at 2000 characters).
Descriptors		CORONARY DISEASE CHLAMYDIA PNEUMONIAE CHLAMYDIA INFECTIONS HISTOCYTOCHEMISTRY ENDARTERECTOMY POLYMERASE CHAIN REACTION ANTIBODIES, BACTERIAL