| Author/Editor | Andjelković, Lea; Šuštaršič, Jasna | |
| Title | Vpliv spola in spolnih hormonov na nastanek akutnih ishemičnih poškodb pri izoliranem podganjem srcu | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Medicinska fakulteta | |
| Publication year | 2001 | |
| Volume | str. 41 | |
| Language | slo | |
| Abstract | Background: Sex might be an important factor in the development of some ischaemic diseases of the heart. The influence of application of sex hormones to experimental animals on the arising of postischaemic heart injury has been only partly investigated. Objective: In our study we tried to assess the influence of subchronical treatment of rats with oestradiol or testosterone on the development of myocardial damage caused by ischaemia/reperfusion. Hypothesis: The sex and sex hormones influence the heart action. After ischaemia/reperfusion less cardiac damage was expected in animals, pretreated with hormones than in control animals. Methods: Experiments were carried out on the isolated hearts of sexually mature rats of both sexes (230-330g). We isolated rat hearts according to Langendorff's method. The hearts were perfused with Krebs-Henseleit solution aerated with carbogen for 30 min and then subjected to 40 min zero-flow ischaemia followed by 55 min reperfusion. The severity of myocardial damage caused by ischaemial reperfusion was assessed by postischaemic changes in the following registe-red parameters: coronary flow, lactate dehydrogenase release rate (LDH), left ventricular pressure (LVP), heart rate and duration of ventricular fibrillation during reperfusion. Results: The coronary flow was higher in the hearts of rats treated with oestradiol or testosterone than in the hearts of control groups. In the hearts of rats treated with oestradiol the postischaemic coronary flow was significantly (p < 0.05) higher than in the hearts of control female group. Postischaemic LDH values were increased in all groups, the highest values were obserVed in the postgravid group. The difference between postgravid and oestradiol group was statistically significant (p < 0.05). Postischaemnic heart rate values were lower than before ischaemia. The highest values were observed in the postgravid group. (Abstract truncated at 2000 characters). | |
| Descriptors | MYOCARDIAL ISCHEMIA ESTRADIOL TESTOSTERONE MYOCARDIAL REPERFUSION INJURY LACTATE DEHYDROGENASE RATS, WISTAR BLOOD PRESSURE HEART RATE VENTRICULAR FIBRILLATION CORONARY VESSELS BLOOD FLOW VELOCITY SEX FACTORS |