Author/Editor		Ocepek, M; Pate, M; Posedi, J
Title		Primernost gojišča BBL MGIT za uporabo v veterinarskem mikobakteriološkem laboratoriju
Translated title		Suitability of BBL MGIT growth medium in veterinary mycobacteriology
Type		članek
Source		Slov Vet Res
Vol. and No.		Letnik 39, št. 2
Publication year		2002
Volume		str. 125-9
Language		slo, eng
Abstract		A comparison has been made between the BBL MGIT growth medium (Becton Dickinson) with 4 conventional solid media (Middlebrook 7H10, Stonebrink, LJ medium with pyruvate and LJ medium with glycerol). Organ specimens obtained from 929 animals were decontaminated with N-acetyl-L-cysteine (NALC)-NaOH using the standard method. Mycobacteria were isolated from 391 of those specimens. The mycobacteria were recovered on MGIT from 323 specimens (82.61 %), on LJ-P from 266 specimens (68.03 %), on Middlebrook 7H10 from 222 (56.78 %), on LJ-G from 251 (64.19 %) and on Stonebrink from 231 specimens (59.08 %). MGIT recovered 40 strains, while only 8 strains were recovered on LJ-P, 6 strains on LJ-G and on Stonebrink and 3 strains on Middlebrook 7H10. The mean detection time of mycobacteria in MGIT was 15.26 (2 - 68) days, on Stonebrink it was 25.33 (5 - 56) days, on Middlebrook 7H10 25.78 (7 - 63) days, on LJ-P 26.56 (5 - 68) days and on LJ-G 30.33 (5 - 64) days. Using MGIT reduced the detection time by 10 days on average and by not using MGIT the results from 40 specimens (4.31 %) would have been false negative.
Summary		Gojišče BBL MGIT (Becton Dickinson) smo primerjali s 4 običajnimi trdimi gojišči (jajčno gojišče s piruvatom (LJ-P), jajčno gojišče z glicerolom (LJ-G), gojišče Stonebrink, gojišče Middelbrook 7H10). 929 vzorcev organov živali smo dekontaminirali po standardni metodi z N-acetyl-L-cysteinom (NALC)-NaOH. Mikobakterije smo izolirali iz 391 vzorcev. Od tega je na gojišču MGIT poraslo 323 (82,61%) sevov, na gojišču LJ-P 266 (68,03%), na gojišču LJ-G 251 (64,19%), na gojišču Stonebrink 231 (59,08%) in na gojišču Middelbrook 7H10 222 (56,78%). Iz 40 vzorcev nam je uspela izolacija le na gojišču MGIT, 8 smo jih izolirali le na gojišču LJ-P, 6 na gojišču LJ-G, 6 na gojišču Stonebrink in le 3 na gojišču Middelbrook 7H10. Na gojišču MGIT so sevi povprečno porasli v 15,26 (2-68) dneh, na gojišču Stonebrink v 25,33 (5-56) dneh, na gojišču Middelbrook 7H10 v 25,78 (7-63) dneh, na gojišču LJ-P v 26,56 (5-68) dneh in na gojišču LJ-G v 30,33 (5-64) dneh. Z gojišči MGIT se je čas detekcije skrajšal v povprečju za 10 dni. Brez uporabe gojišč MGIT bi bilo 40 (4,31 %) vzorcev lažno negativnih.
Descriptors		MYCOBACTERIUM CULTURE MEDIA, CONDITIONED TUBERCULOSIS MYCOBACTERIUM AVIUM MYCOBACTERIUM TUBERCULOSIS