Author/Editor		Kandus, A
Title		Adsorpcija komplementnih fragmentov na različnih dializnih sistemih z akrilonitrilnimi membranami
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		1991
Volume		str. 64
Language		slo
Abstract		In these prospective clinical investigation it was attempted: 1) to test the hypothesis that various acrylonitrile membranes (AN69) can adsorb a larger quantity of complement fragments C3a and C5a than they can produce (Investigation 1); 2) to study the adsorption and the production of C3a and C5a on the surface of the outlet tube of the cuprophane membrane hemodialyzer (investigations 2); 3) to study the possibility of further production of C3a and C5a while blood was passing through an additional cuprophane membrane hemodialyzer of the same type (investigation 3); Investigation 1 was performed during a dialysis of 4 hours duration in 8 patients, treated with chronic periodic hemodialysis. Their blood flowing from a cuprophane membrane hemodialyzer passed through an additional AN69 hemodialyzer in which no hemodialysis took place. This investigation was conducted in 3 parts. In each of this parts one cuprophane membrane hemodialyzer of the same type, and one of three different AN69 hemodialyzers (Filtral AN69, H 12 10 and Biospal 3000 S) were used. In blood samples taken from the inlet and outlet tube at the 15th, 60th and 20th min. of hemodialysis, plasma C3a and C5a concentrations were measured by using a radioimmunoassay method. In the blood which passed through the AN69 hemodialyzer the concentration of C3a was significantly decreased. This decrease, suggesting and adsorption on the AN69, was maximal (by 54 percent to 65 percent) at the 15th min. and minimal (by 18 percent to 53 percent) at the 240th min. of hemodialysis. The decrease of plasma C5a concentration was less pronounced, though significant; the only exception was in the hemodialyzer H 12 10 at the 240th min. of hemodialysis. The calculated adsorbed amount of C3a on the AN69 was 15,000 to 46,000 micro g. The results support the hypothesis, that various AN69 can adsorb more C3a, and probably more C5a, than they produce.(trunc.)
Descriptors		HEMODIALYSIS HEMODIALYSIS SOLUTIONS ACRYLONITRILE COMPLEMENT 3A COMPLEMENT 5A PROSPECTIVE STUDIES KIDNEY FAILURE, CHRONIC MEMBRANES, ARTIFICIAL RADIOIMMUNOASSAY HEMATOCRIT