Author/Editor     Bizimoska, Saška
Title     Merjenje pO2 v ustni sluznici podgan po nanosu hiperemikov z EPR oksimetrijo
Type     monografija
Place     Ljubljana
Publisher     Medicinska fakulteta
Publication year     2001
Volume     str. 41
Language     slo
Abstract     BACKGROUND. Ointments acting as vehicles for local delivery of drugs to the oral mucosa need to have accellent adhesive properties due to the flow of saliva. Such ointments are Orabase, Carbopol and polymethyl methacrylate. Many different researches on previously mentioned ointments have been made so far, mostly in vitro, where polymethyl methacrylate has showed the best results on bioadhesion. AIM. Our research set to compare three adhesive ointments (Orabase, Carbopol, polymethyl methacrylate) in vivo. We added a hyperemic to each of the chosen ointments, thereby we caused a change in local pO2 in oral mucosa, which we then followed by EPR. HYPOTHESIS. We tested these two hypotheses: Hypothesis 1: polymethyl methacrylate will make, in vivo as well, the greatest and longest change in pO2 in oral mucosa possible; Hypothesis 2: different levels of pO2 in oral mucosa can be reached by changing the concentration of the hyperemic. METHODS. Our research was conducted on female Wistar rats. We compared three ointments with bioadhesive properties (Orabase, Carbopol, polymethyl methacrylate) and the ointment without such properties (Miglyol). We added the hyperemic (benzil-nicotinate) to each of them and then applied 0,05 ml of the 2% solution to rats' buccal mucosa, where we had previously put a paramagnetic probe. Rats in control groups were given only ointments without the hyperemic. After the application we followed the time-course of the pO2 changes in oral mucosa with the EPR spectrometer, adjusted for operating at 1,2 GHz. The ointment which made possible for the hyperemic to cause the greatest change in pO2 (polymethyl-methacrylate) was chosen to test the second hypothesis. The position of the paramagnetic probe and changes in its surroundings were also assessed histologically. Results were analyzed using the analysis of variance followed by post hoc Tukey's test. (Abstract truncated at 2000 characters).
Descriptors     MOUTH MUCOSA
HYPEREMIA
ADHESIVES
POLYMETHACRYLIC ACIDS
RATS, WISTAR
OINTMENTS
ELECTRON SPIN RESONANCE SPECTROSCOPY
OXYGEN