Author/Editor		Smerdu, Vika; Eržen, Ida
Title		Dynamic nature of fibre-type specific expression of myosin heavy chain transcripts in 14 different human skeletal muscles
Type		članek
Source		J Muscle Res Cell Motil
Vol. and No.		Letnik 22
Publication year		2001
Volume		str. 647-55
Language		eng
Abstract		The main goal of this study was to find out, whether the appearance of fibres without evident myosin heavy chain (MyHC) transcript expression (negative fibres) implies the existence of additional MyHC transcripts in human muscle fibres. Fourteen different skeletal muscles were analysed also to verify how MyHC transcript expression matches histochemical phenotypes of fibres. For this purpose, the expression of (3-slow, 2a and 2x MyHC transcripts, demonstrated by in suu hybridisation technique, was analysed within type I, IIC, IIA, IIAX and IIX fibres, determined according to the activity of myofibrillar ATPase. Additionally, MyHC isoform expression was immunohistochemically demonstrated and metabolic profiles of negative fibres were estimated. From a total of 4444 muscle fibres analysed, only 0.8% of fibres were negative, among them type I prevailed, the remainder were type IIA and IIX fibres. The majority of fibres expressed only beta, 2a and 2x MyHC transcripts and they mostly matched type I, IIA and IIX fibres respectively, but two minor hybrid fibre groups ((3/2a and 2ax) exhibited variable histochemical phenotype. The infrequency, the prevailing oxidativž-glycolytic metabolic profile of negative type I fibres and frequent co-appearance with transitional type IIC fibres imply that the negative fibres rather result from fibre type transition than express an additional slow or even 2b MyHC transcripts. The appearance of hybrid and mismatched fibres additionally indicates that fibre type transition occurs also in presumably normal skeletal muscles, what enables the muscles to tune even with minimal changes in mechanical demands.
Descriptors		MUSCLE FIBERS, FAST-TWITCH MUSCLE FIBERS, SLOW-TWITCH MUSCLE, SKELETAL MYOSIN HEAVY CHAINS AGE FACTORS IMMUNOHISTOCHEMISTRY PHENOTYPE RNA, MESSENGER