Author/Editor		Haegerstrand, Henry; Iglič, Aleš; Bobrowska-Haegerstrand, Malgorzata; Lindquist, Christer; Isomaa, Boris; Eberg, Stefan
Title		Amphiphile-induced vesiculation in aged hereditary spherocytosis erythrocytes indicates normal membrane stability properties under non-starving conditions
Type		članek
Source		Mol Membr Biol
Vol. and No.		Letnik 18
Publication year		2001
Volume		str. 221-7
Language		eng
Abstract		Aged HS erythrocytes with a defined primary defect in band 3 protein or ankyrin were incubated with amphiphiles (detergents) at sublytic concentrations (37degreesC, 60 min) or glucose-starved (37degreesC, 24 h). In line with previous studies, the release of AChE (exovesicles) from HS erythrocytes during glucose-starvation was significantly higher (71%) compared to that from control erythrocytes (1%). Control and HS cells responded, however, similarly to amphiphile-treatment (non-starving conditions). Amphiphiles induced similar types of shape alterations and a similar amount of AChE release (14-15%). Furthermore, the size and shape of amphiphile-induced exo- and endovesicles released from control and HS erythrocytes were similar. The results suggest that the stability properties of the membrane are not seriously disturbed in aged HS erythrocytes under nonstarving conditions.
Descriptors		CELL AGING CELL MEMBRANE ERYTHROCYTES SPHEROCYTOSIS, HEREDITARY OSMOTIC FRAGILITY GLUCOSE FOOD DEPRIVATION CELL SIZE