Author/Editor		Dubničkova, Martina; Bpbrowska-Haegerstrand, Malgorzata; Soederstroen, Thomas; Iglič, Aleš; Haegerstrand, Henry
Title		Gemini (dimeric) surfactant perturbation of the human erythrocyte
Type		članek
Source		Acta Biochim Pol
Vol. and No.		Letnik 47, št. 3
Publication year		2000
Volume		str. 651-60
Language		eng
Abstract		We studied the ability of di-cationic gemini surfactantsdi (amphiphiles), i.e. 1,4-butanediaxnmonium N,N-dialkyl-N,N,N,N-tetramethyl bromides (Di-Cm-di-QAS (s = 4), where m = 8,11,13,16 and s = the number of alkyl groups in the spacer) to induce shape alteration, vesiculation, haemolysis and phosphatidylserine exposure in human erythrocytes, and to protect erythrocytes against hypotonic haemolysis. At high sublytic concentrations the Di-Cm-di-QAS (s = 4) amphiphiles rapidly induced echinocytic (spiculated) shapes and a release of exovesicles, mainly in the form of tubes, from the cell surface. Following 60 min incubation erythrocytes were sphero-echinocytic and a few cells with invaginations/endovesicles were observed. No phosphatidylserine exposure was detected. The haemolytic potency increased with an increase of the alkyl chain length. At sublytic concentrations the Di-Cm-di-QAS (s = 4) amphiphiles protected erythrocytes against hypotonic haemolysis. (Abstract truncated at 2000 characters)
Descriptors		SURFACE-ACTIVE AGENTS ERYTHROCYTES STRUCTURE-ACTIVITY RELATIONSHIP MICROSCOPY, ELECTRON PHOSPHATIDYLSERINES HEMOLYSIS CELL SIZE