Author/Editor		Tomšič, Martin
Title		Model regeneracije prekinjenih perifernih živcev v vodilu z elektromagnetnim poljem
Type		monografija
Place		Ljubljana
Publisher		Fakulteta za elektrotehniko
Publication year		2001
Volume		str. 156
Language		slo
Abstract		Electromagnetic fields have been shown to affect neurite outgrowth. The present study evaluated the effect of electromagnetic fields on regeneration of sensory axons of peripheral sural nerve in vivo through different nerve guides (nerve guidance channels, nerve regeneration chambers). The problem is a part of the efforts to improve poor results in peripheral nerve repair. Nerve guides made of metal and silicone rubber tubes were sutured between proximal and distal stumps of the injured sural nerve in rats. Regeneration of rats' sensory axons into the distal stump of sural nerve was examined 6 weeks after surgery using electrophysiology and histomorphometry. Stainless steel tubes contained nerve bridges (cables) with significantly less myelinated and non-myelinated axons than silicone rubber tubes. It seems that regeneration of injured axons through electrically conductive and ferromagnetic nerve guide is hindered. Effect of direction and intensity of stationary direct current electric field was studied subsequently by implantation of silicone rubber tubes with differently arranged electrodes. Electromagnetic fields imposed by electrodes inserted into nerve guides significantly changed nerve growth across the 3mm gap. The total electric current was 1microA and 0.3micro.A. The distal anode hindered regeneration, while the effect of the distal cathode was not detected. Results of these experiments with localized of exogenous currents indicate that some stimuli can accelerate the growth of neurities and the direction of their growth my coincide with electromagnetic fields. The present findings support the hypothesis that axonal outgrowth in vivo is influenced by electromagnetic fileds.
Descriptors		SURAL NERVE NERVE REGENERATION AXONS ELECTROMAGNETIC FIELDS NEURAL CONDUCTION RATS