| Author/Editor | Glaser, Marjana | |
| Title | Proliferacijska sposobnost hematopoetskih matičnih stanica kod kroničnog bubrežnog zatajenja | |
| Type | monografija | |
| Place | Zagreb | |
| Publisher | SveučIlište u Zagrebu, Medicinski fakultet | |
| Publication year | 2002 | |
| Volume | str. 112 | |
| Language | cro | |
| Abstract | Chronic renal failure (CRF) is accompanied by numerous complications including hematologic, and among these anemia is the most obvious. It is assumed that apart from the known causes such as the lack of erythropoietina (EPO), the entire hematopoiesis is also disturbed: Numerous substances are involved in the inhibition of erythropoiesis. However, it remains unelucidated at which level and in which part of hematopoietic stem cells and by which mechanism inhibition occurs and whether this phenomenon is the exclusive result of the lack of EPO. Therefore the aim of this study was to verify the hypothesis that, apart from the lack of EPO, other agents also play a part in the pathogenesis of anemia in CRF, and whether this disturbance affects the entire hematopoiesi and at a higher hierarchic level of stem cells than that of erytrocytopoiesis. So the basic aim of the study was to investigate the clonogenic and proliferative capacity not merely of stem cells for erythrocvtopoiesis (BFU E), but the clonogenic and proliferative capacity of cells whose development is not affected by EPO (granulocyte-monocyte colony forming unit - CFU GM), or of several types of cells (colony forming unit granulocyte-erythrocyte-monocyte-megakaryocyte CFU GEMM) whose development is also not affected by EPO. The aim was to test their functional capacity to respond to the regulatory stimulus in the culture. In this way we wanted to establish to what extent and which part of hematopoiesis is inhibited in CRF patients as a possible cause of anemia. The specific aims of this study were: to determine the clonogenic and proliferative capacity of the rnultipotent cell for myelopoiesis (CFU GEMM), the target stem cell for granulocytopoiesis and monocytopoiesis (CFU GM) and the stem cell for erythrocytopoiesis (BFU E), as well as to determine EPO concentration and evaluate its relation to the proliferative capacity of stem cells. (Abstract truncated at 2000 characters) | |
| Descriptors | KIDNEY FAILURE, CHRONIC HEMATOPOIETIC STEM CELLS COLONY-FORMING UNITS ASSAY KIDNEY FUNCTION TESTS HEMATOLOGIC TESTS UREA CREATININE URIC ACID SODIUM POTASSIUM CALCIUM ERYTHROCYTE COUNT LEUKOCYTE COUNT PLATELET COUNT IRON FERRITIN BLOOD SEDIMENTATION ERYTHROPOIETIN |