Author/Editor		Matičič, Mojca
Title		Prisotnost provirusne HIV-1 DNA in protiteles proti HIV-1 v gingivalni tekočini HIV-1 seropozitivnih oseb v Sloveniji
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		1997
Volume		str. 78
Language		slo
Abstract		Epidemiological studies suggest that human immunodeficiency virus (HIV) is not spread by casual contacts, but rather by exposure to contaminated blood or blood products, by sexual contact with an infected person and from infected mother to offspring. A considerable amount of concern, however, still exists regarding a possible role of saliva in transmitting HIV in some particular reported cases. In case of infection or mechanical irritation of gingiva, saliva might harbour gingival fluid which is excreted at dento-gingival interface. Since in HIVinfected persons the prevalence of oral pathology and periodontal disease is frequent, the excretion of mononuclear cells enriched gingival fluid is increased. The aim of our study was to detect proviral HIV-1 DNA in gingival fluid of 35 persons, previously determined as HIV-1 seropositive, by means of direct and indirect methods, and to Iook for its relationship with their clinical, laboratory and therapeutic characteristics. Gingival fluid samples were collected by paper points using intracrevicular method and analysed for the presence of anti HIV-1/2 antibodies by slightly modified standardised enzyme immuno assay (for testing of urine, saliva and blood spot eluates) and confirmatory Western blot test. To detect proviral HIV-1 DNA a slightly modified standardised polymerase chain reaction kit was used. Proviral HIV-1 DNA was detected in 17 out of 35 gingival fluid samples of HIV-1 seropositive persons expressing most various stages of HIV disease and oral pathology, with wide range of serum HIV-1 RNA viral load and different variations in use of antiretroviral therapy. It was found only in patients with a CD4+ cell count < 500/mm3 and was significantly more often detected in persons with higher plasma viral load (p= 0.03). (Abstract truncated at 2000 characters).
Summary		Epidemiološke študije so pokazale, da se virus človeške imunske pomanjkljivosti (HIV, okr. angl. human immunodeficiency virus) ne prenaša ob vsakodnevnih mesebojnih stikih, temveč pri spolnem stiku z okuženo osebo, pri parenteralnem stiku z okuženo krvjo ali krvnimi pripravki, ter z okužene matere na plod oz. novorojenca. Slina globalno epidemiološko ni zelo pomembna pri prenosu okužbe s HIV, še vedno pa niso pojasnjeni nekateri izjemni načini okužbe, pri katerih bi le-ta lahko bila udeležena. Sestavni del sline je lahko tudi gingivalna tekočina, ki ob okužbah ali mehaničnih poškodbah dlesne nastaja v dento-gingivalnem predelu. Pri osebah, okuženih s HIV, je zaradi pogosto prisotnih bolezni ustne sluznice in obzobnih tkiv izločanje gingivalne tekočine, obogatene z veliko mononuklearnih celic, povečano. Namen raziskave je bil neposredno in posredno ugotoviti prisotnost HIV v gingivalni tekočini klinično, imunološko in virološko opredeljenih HIV-1 seropozitivnih oseb in pojasniti vpliv različnih kliničnih, laboratorijskih in terapevtskih dejavnikov na pojavljanje provirusne HIV-1 DNA v gingivalni tekočini. Vzorce gingivalne tekočine smo odvzeli s papirnatimi šilci z intrasulkusno metodo. Za ugotavljanje specifičnih protiteles proti HV-1/2 v vzorcih
Descriptors		HIV INFECTIONS HIV-1 GINGIVAL CREVICULAR FLUID HIV SEROPOSITIVITY DNA, VIRAL ANTIBODIES, VIRAL RNA, VIRAL MOUTH DISEASES IMMUNOENZYME TECHNIQUES BLOTTING, WESTERN POLYMERASE CHAIN REACTION