Author/Editor     Turk, Martina; Plemenitaš, Ana
Title     The HOG pathway in the halophilic black yeast Hortea werneckii: isolation of the HOG1 homolog gene and activation of HwHog1p
Type     članek
Source     FEMS Microbiol Lett
Vol. and No.     Letnik 216
Publication year     2002
Volume     str. 193-9
Language     eng
Abstract     The mitogen-activated protein kinase (MAPK) Hoglp plays an essential role in the yeast hyperosmotic response. A homolog of the HOG1 gene was isolated from the halophilic black yeast Hortaea werneckii encoding a putative 359 amino acid protein, HwHoglp, with high homology to Saccharomyces cerevisiae Hoglp and to other eukaryotic Hoglp homologs. HwHoglp contains a TGY motif within a protein kinase catalytic domain and a C-terminal common docking (CD) motif. Its activation by increased salinity is regulated at the posttranscriptional level. HwHoglp is located on the plasma membrane under nonstress conditions. Upon increased external salinity it is translocated from the membrane, presumably to the nucleus.
Descriptors     YEASTS
PROTEIN-SERINE-THREONINE KINASES
CELL MEMBRANE
ASCOMYCETES
SIGNAL TRANSDUCTION
DNA, FUNGAL
BLOTTING, WESTERN
OSMOTIC PRESSURE
GLYCERIN
CLONING, MOLECULAR
BASE SEQUENCE
BLOTTING, SOUTHERN