Author/Editor     Herman, Darja; Narat, Mojca; Benčina, Dušan
Title     Dokazovanje protiteles proti patogenim mikroorganizmom v tekočinah kokošjega zarodka
Translated title     Detection of antibodies to pathogenic microorganisms in the chicken embryo's fluids
Type     članek
Source     Zb Bioteh fak Univ Edvarda Kardelja Ljublj, Kmet, Supl
Vol. and No.     Letnik 78, št. 2
Publication year     2001
Volume     str. 127-35
Language     slo
Abstract     Certain avian pathogens can be transmitted in chickens via hatching eggs. Specific maternal antibodies appearing in the egg yolk and egg white are able to protect embryo against a variety of vertically transmitted bacterial and viral pathogens. Immunoenzyme tests using different monoclonal antibodies (mAb) to chicken immunoglobulins G (IgG), IgA and IgM were used for detection of antibodies to Mycoplasma synoviae, M. gallisepticum, Ornithobacterium rhinotracheale and Newcastle disease virus in the allantoic fluid (ALF), amniotic fluid (AMF), bile and serum of chicken embryos on different stages of the embryonic development. In the ALF of 7-day-old embryos IgG antibodies to M. synoviae were demonstrated for the first time in this study. Certain samples contained also IgG antibodies to M. gallisepticum and Newcastle disease virus. In addition, IgA, and IgM were also found in the ALF. It seems that transfer of IgA and IgM from the egg white to ALF is selective. In AMF specific IgG antibodies to M. synoviae were detected on day 7 of the embrionic development. From 12th day of the embryogenesis onward levels of IgA, IgG and IgM in AMF were apparently increased. Specific IgA and IgG antibodies to M. synoviae and O. rhinotracheale were detected in bile of 13-day-old embryos. In the blood, which was taken from the chorioallantoic veins from the 13th to 16th day of the embryogenesis, IgG and IgA were found.
Summary     Pred patogenimi mikroorganizmi, ki se prenašajo prek valilnih jajc, kokošji zarodek ščitijo protitelesa matere v rumenjaku in beljaku. Z uporabo imunoencimskih testov in monoklonskih protiteles (mAb) proti kokošjim imunoglobulinom razredov G (IgG), IgA in IgM smo ugotavljali prisotnost specifičnih protiteles proti Mycoplasma synoviae, M. gallisepticum, Ornithobacterium rhinotracheale in virusu atipične kokošje kuge v alantoisni tekočini (ALT), amnionski tekočini (AMT), žolču in krvi kokošjih zarodkov na različnih stopnjah embrionalnega razvoja. Že 7. dan embrionalnega razvoja je večina zarodkov imela v ALT specifična protitelesa IgG proti M synoviae, nekateri zarodki pa tudi protitelesa IgG proti M. gallisepticum in virusu atipične kokošje kuge. V ALT so bila pri nekaterih zarodkih prisotna tudi protitelesa razreda IgA in IgM. Protitelesa IgA in IgM so se najverjetneje prenesla iz beljaka v ALT. V AMT smo specifična protitelesa IgG proti M. synoviae dokazali 7. dan embrionalnega razvoja. Količina IgA, IgG in IgM v AMT se je močno povečala po 12. dnevu embrionalnega razvoja. Specifična protitelesa lahko pridejo tudi v žolč, kjer smo protitelesa IgA in IgG protiM. synoviae m O. rhinotracheale dokazali pri 13 dni starih zarodkih. Tudi v vzorcih krvi, vzetih iz alantoisnega krvnega obtoka od 13. do 16. dne embrionalnega razvoja, smo dokazali IgG in IgA.
Descriptors     CHICK EMBRYO
ANTIBODIES, BACTERIAL
ANTIBODIES, VIRAL
ANTIBODIES, MONOCLONAL
IMMUNOENZYME TECHNIQUES
IGG
IGA
IGM
MYCOPLASMA
ORNITHOSIS
NEWCASTLE DISEASE
ALLANTOIS
AMNIOTIC FLUID