Author/Editor		Malovrh, T; Jurca, J; Gruntar, I; Kotnik, V
Title		Lymphocyte proliferation assay in response to Borrelia burgdorferi s. l. in experimentally infected C57b1/6 mice
Translated title		Test blastnega preoblikovanja limfocitov ob odzivu miši C57b1/6 pri eksperimentalni okužbi z bakterijo Borrelia burgdorferi s. l.
Type		članek
Source		Slov Vet Res
Vol. and No.		Letnik 39, št. 3-4
Publication year		2002
Volume		str. 189-99
Language		eng
Abstract		Inbred C57b1/6 mice were intraperitoneally infected with Borrelia afzelii, Borrelia garinii and Borrelia burgdorferi sensu stricto (s.s.) spirochaetes and tested in vitro for antigen-specific T-cell responses. Sonicated preparations of these washed spirochaetes were potent cell-activators capable of stimulating polyclonal proliferation after 72 h of culture, while increasing the incubation time to 120 h provoked specific cell-mediated responses. As revealed by an [3H]thymidineincorporation assay, in vitro antigen-specific proliferation could be induced in isolated murine splenocytes previously sensitised to B. burgdorferi sensu lato (s.l.), although not in those from control mice. Moreover, in mice presensitised to B. burgdorferi s.l., detectable cell-mediated responses could only be induced with antigen preparations derived from a corresponding species and not with those obtained from other Borrelia genospecies. The current study indicates that the B. burgdorferi s.l. antigen-specific response should also be taken into account in the accurate diagnostics of Borrelia infections in humans.
Summary		Pri miši sokrvnega seva C57b1/6 smo po okužbi z bakterijami Borrelia afzelii, Borrelia garinii in Borrelia burgdorferi sensu stricto (s.s.) preverjali antigensko specifičen T-celični imunski odziv v in vitro pogojih. Pripravek spranih in ultrazvočno razbitih spirohet je nespecifičen celični aktivator, ki sproži poliklonsko aktivacijo limfocitov po 72-urni inkubaciji v kulturi, s podaljšanjem inkubacijskega časa na 120 ur pa sproži specifičen celični odgovor. Izolirane limfocitne celice iz vranic miši, okuženih z B. burgdorferi sensu lato (s.l.), se odzovejo na antigensko spodbudo s proliferacijo v in vitro pogojih, merjeno s testom vgradnje [3H)timidina, v primerjavi s celicami, pridobljenimi iz kontrolnih, neokuženih miši. Poleg tega je pri miših, ki so bile okužene z B. burgdorferi s.l, možno določiti specifičen celični imunski odziv samo s pripravki antigena, ki izvirajo iz iste vrste borelije, s katero so bile miši okužene. V opisani študiji tako nakazujemo, da lahko pričakujemo pri B. burgdorferi s.l. specifičen celični imunski odziv tudi pri ljudeh, okuženih z različnimi vrstami borelij.
Descriptors		BORRELIA BURGDORFERI ANTIBODIES, BACTERIAL LYMPHOCYTE TRANSFORMATION MICE, INBRED C57BL BORRELIA