Author/Editor		Kožar-Logar, Jasmina; Malej, Alenka; Franko, Mladen
Title		Hyphenated high perfomance liquid chromatography-thermal lens spectrometry technique as a tool for investigations of xanthophyll cycle pigments in different axonomic groups of marine phytoplankton
Type		članek
Source		Rev Sci Instrum
Vol. and No.		Letnik 74, št. 1
Publication year		2003
Volume		str. 776-8
Language		eng
Abstract		Hyphenated high performance liquid chromatography-thermal lens spectrometry (HPLC-TLS) was applied to the determination of carotenoids in four phytoplankton species. Satisfactory separation of diadinoxanthin, diatoxanthin, and other carotenoids was achieved by isocratic HPLC elution providing 40-80 times lower LOD, compared to UV Vis detector. Besides the interconversion of diadinoxanthin to diatoxanthin, changes of other minor carotenoids under different light conditions were possible to follow. The isocratic HPLC-TLS provides sufficient sensitivity and selectivity for studies of photochemical processes in marine phytoplankton involving pigments, which occur in traces.
Descriptors		CHROMATOGRAPHY, HIGH PRESSURE LIQUID ALCOHOL, METHYL ACETONE AMMONIUM COMPOUNDS TEMPERATURE REFRACTOMETRY