Author/Editor		Strojan-Fležar, Margareta; Us-Krašovec, Marija
Title		Slikovna citometrija v onkologiji
Translated title		Image cytometry in oncology
Type		članek
Source		Med Razgl
Vol. and No.		Letnik 41, št. 4
Publication year		2002
Volume		str. 313-20
Language		slo
Abstract		Image cytometry is a static, quantitative analytical method used for objective measurement of numerous cell characteristics on glass slides. Specifically, it makes it possible to measure the DNA content and the nuclear texture features of single cells, either cancerous or normal, or of single cells belonging to an undefined stage of neoplastic transformation. Monolayered single cell slides are required for optimal analysis using an image cytometer. Cell preparations are stained according to the Feulgen method for stoichiometric staining of DNA in the cell nucleus. An image cytometer is an optical electronic system equipped with high-powered computers. Numerous nuclear texture features are calculated from nuclear images, which are acquired via image cytometer. New image cytometric features of malignant cells could be used as additional prognostic factors, with standard clinical and histopathologic factors that are commonly used to predict the course of the disease and the patient's response to treatment. Above all, we are hoping that image cytometric analysis of subvisual changes of chromatin in normal cells will provide new tests for cancer detection at preclinical stages.
Summary		Slikovna citometrija je statična kvantitativna analitična metoda, ki omogoča merjenje številnih lastnosti celic na objektnem stekelcu. S slikovnim citometrom lahko podrobno analiziramo količino DNA ter strukturo in razporeditev jedrnega kromatina v posamični celici, bodisi rakavi ali normalni, oziroma v celici, ki pripada neki vmesni stopnji med neoplastično preobrazbo. Za optimalno analizo s slikovnim citometrom potrebujemo celične vzorce, v katerih so celice nanešene v enem sloju in med seboj dovolj razmaknjene. Preparate barvamo po Feulgnovi metodi, ki omogoča stehiometrično barvanje DNA v jedru. Na podlagi jedrnih slik, ki jih posname slikovni citometer (optično elektronsko računalniški sistem) na celičnem preparatu, izračunamo številne jedrne značilnosti. Nove slikovnocitometrične značilnosti rakavih celic omogočajo dodatno opredelitev klasičnih kliničnih in histopatoloških dejavnikov poteka bolezni in odziva na zdravljenje. Predvsem pa upamo, da nam bo slikovnocitometrična analiza subtilnih sprememb kromatina na normalnih celicah omogočila odkrivati raka pri bolnikih, ki še ne kažejo kliničnih znakov bolezni.
Descriptors		NEOPLASMS IMAGE CYTOMETRY PLOIDIES