Author/Editor		Zavašnik-Bergant, Valentina
Title		Vloga oblike p41 invariantne verige in cistatina C pri uravnavanju cisteinskih proteaz v antigen predstavitvenih celicah
Translated title		Role of p41 isoform of invariant chain and cystatin C in regulating cysteine proteases in antigen-presenting cells
Type		monografija
Place		Ljubljana
Publisher		Mentor: Kos J
Publication year		2003
Volume		str. 108
Language		slo
Abstract		Activation of CD4+ T cells is an important regulatory step in the adaptive immune response to an antigen, In addition to processing endocytosed or phagocytosed antigens, the capacity of professional antigen-presenting cells (APC), such as dendritic cells, macrophages and B cells, to activate CD4+ T cell response relies on their ability to mature MHC class II molecules, to facilitate loading of antigenic peptides on them and to transport these complexes to the cell surface for recognition by T-cell receptors, Cysteine and aspartyl proteases appear to be implicated, either in processing antigenic peptides or in stepwise degradation of invariant chain (li), a transmembrane chaperone protein that complexes with MHC class II molecules. Regulation of protease activity by endogenous protein inhibitors (protease propeptides, cystatins, thyropins) plays an important role in modulating antigen presentation in APC, The p41 isoform of invariant chain (p41 li) and cystatin C were suggested to modulate the proteolytic capacity of cysteine proteases cathepsins L and S,In order to study the distribution of two different li isoforms (inhibitory p41 li and non-inhibitory p31 li) in lymphatic tissue, two different monoclonal antibodies (mAb)were prepared and characterized; 2C12 mAb, recognising inhibitory (thyropin-like) p41 fragment on the lumenal C-terminal end of p41 li, and 5F6 mAb, recognising an epitope on the non-inhibitory portion of li, In our study the distribution and co-localization of cathepsins S, L and H with their potential inhibitors (p41 li and cystatin C) have been determined and their possible interactions addressed in non-invaded human lymph nodes, lung tissue and tumours and, more precisely, at the subcellular level in isolated monocytes. (Abstract truncated at 2000 characters).
Descriptors		LYMPH NODES LUNG NEOPLASMS CATHEPSINS ANTIGEN-PRESENTING CELLS CYSTATINS MICE, INBRED BALB C HYBRIDOMAS ANTIBODIES, MONOCLONAL CD4-POSITIVE T-LYMPHOCYTES DENDRITIC CELLS HISTOCOMPATIBILITY ANTIGENS CLASS II ELECTROPHORESIS, POLYACRYLAMIDE GEL IMMUNOHISTOCHEMISTRY MICROSCOPY, CONFOCAL BLOTTING, WESTERN ENZYME-LINKED IMMUNOSORBENT ASSAY MONOCYTES MICROSCOPY, ELECTRON