Author/Editor		Petelin, M
Title		Vloga bakterije Porphyromonas gingivalis na razvoj vnetja pljuč pri miših
Translated title		The role of Porphyromonas gingivalis in pathogenesis of pulmonary inflammation in mice
Type		članek
Source		Zobozdrav Vestn
Vol. and No.		Letnik 58, št. 1
Publication year		2003
Volume		str. 3-9
Language		slo
Abstract		Background: The anaerobic bacterium Porphyromonas gingivalis accounts for a high percentage of microorganisms present in periodontal pockets of patients with advanced periodontal disease. Several studies have documented that nursing home subjects have an increased risk for aspiration pneumonia caused by oral pathogens. Materials and Methods: Pulmonary inflammation in mice was produced by intratracheal inoculation with the extract of Porphyromonas gingivalis. The local and systemic response to the inflammation was studied by histological examination of lung tissue and measurement of the levels of TNF-alpha and soluble TNF receptors in serum and lungs. Results: A day after inoculation, neutrophil infiltration was visible mainly in peribronchial areas. Three days later, neutrophils were present in most alveoli but there was no evidence of pulmonary parenchymal necrosis. Two hours after inoculation, TNF-alpha increased in lung tissue but remained undetectable in serum. The level of soluble TNF-2 receptor in lung tissue increased appreciably a day after inoculation and remained elevated for three days. On day 7 following inoculation, the histological appearance of lung tissue was back to normal and the levels of TNF-alpha and soluble TNF-2 receptor returned to normal values. Conclusion: Soluble TNF-2 receptor seems to play a key role in reducing the levels of TNF-alpha in the inflamed lung and thereby prevents its elevation in blood.
Summary		Izhodišča: Anaerobna bakterija Porphyromonas gingivalis zavzema pri napredujoči parodontalni bolezni visok odstotek mikroorganizmov v obzobnih žepih. Številne študije dokazujejo, da starejši lečeči bolniki pogosteje zbolevajo za aspiracijsko pljučnico, ki jo verjetno povzročijo anaerobne bakterije iz ustne votline. Materiali in metode: Da bi ugotovili lokalni in sistemski odgovor organizma pri pljučnem vnetju, smo mišim skozi sapnik v pljuča vbrizgali ekstrakt bakterije Porphyromonas gingivalis. V pljučih in krvnem serumu smo določali koncentracije citokina TNF-alfa in topnih receptorjev TNF. Pljučno tkivo smo pregledali tudi histološko. Rezultati: En dan po vbrizganju bakterijskega ekstrakta se je pojavila infiltracija nevtrofilcev v pljučnih mešičkih predvsem ob bronhiolih, tri dni kasneje pa smo ugotovili nevtrofilce v večini pljučnih mešičkov, brez nekroze intersticija. V prvih dveh urah po vbrizganju bakterijskega ekstrakta se je v pljučih, ne pa v serumu, povečala koncentracija TNF-alfa. Prvi dan po vbrizganju se je v pljučih občutno povišala raven topnega receptorja TNF-2 in ostala povišana tri dni. Sedmi dan sta bili histološka slika pljučnega tkiva ter koncentracije TNF-alfa in receptorjev na normalnih vrednostih. Zaključek: Topni receptor TNF-2 je verjetno ključnega pomena pri zniževanju vsebnosti TNF-alfa v vnetih pljučih in s tem preprečuje njegovo povišanje v krvi.
Descriptors		PNEUMONIA, ASPIRATION PORPHYROMONAS GINGIVALIS MICE TUMOR NECROSIS FACTOR RECEPTORS, TUMOR NECROSIS FACTOR