Author/Editor		Nemeth, L; Erman, A; Štiblar-Martinčič, D
Title		Kvalitativna in polkvantitativna analiza odziva zobne pulpe na preparacijo in jedkanje kavitete pri podganah
Translated title		Qualitative and semiquantitative analysis of dental pulp response to cavity preparation and acid etching in rats
Type		članek
Source		Zobozdrav Vestn
Vol. and No.		Letnik 58, št. 1
Publication year		2003
Volume		str. 11-9
Language		slo
Abstract		Background: Opinions on the influence of acid etching on the dental pulp are divided. The purpose of the study was to compare the response of the dental pulp to cavity preparation when acid etching was used and when etching was omitted. Materials and methods: Class V cavities on the mesial surface of the first upper molar in 28 Wistar rats were prepared for restoration. In each animal, the cavity in the right molar was etched with acid, rinsed with saline, gently dried with air and restored with Cavit. The cavity in the contralateral, left tooth was only rinsed with saline, dried with air and restored with Cavit. The left and right second molars served as control. The animals were sacrificed 5 min, 6 h, 24 h and 72 h postoperatively. Histological and ultrathin sections of the treated teeth were examined by light and electron microscopy. Qualitative and semi-quantitative analysis was performed. Results: Comparison of sections from the etched and unetched teeth showed that 5 minutes postoperatively, the odontoblastic layer was more disorganized beneath the etched cavities; 6 and 24 hours postoperatively, fewer odontoblasts were visible and hyperaemia of the pulp was more extensive beneath the etched cavities; 72 hours postoperatively, there was less evidence of repair beneath the etched cavities, but the inflammatory reaction in the pulp was equally pronounced in both sets of teeth. Ultrastructural analysis revealed apoptosis to be a possible form of cell death after both procedures. Conclusion: Acid etching of the cavity causes additional injury to odontoblasts. Inflammation in the dental pulp appears simultaneously with dentinogenesis. The study should be complemented by analysis of bacteria present in cavities.
Summary		Izhodišče: Stroka si ni popolnoma enotna o tem, kako jedkanje kavitete vpliva na zobno pulpo. V naši raziskavi smo želeli primerjati odziv zobne pulpe na preparacijo kavitete in njeno jedkanje ter na preparacijo brez jedkanja. Materiali in metode: Na mezialni ploskvi prvih zgornjih kočnikov 28-ih podgan seva Wistar smo spreparirali kaviteto V. razreda. Kaviteto na desnem kočniku smo jedkali, izprali s fiziološko raztopino, osušili in zaprli s polnilom Cavit. Kaviteto na levem kočniku smo samo izprali s fiziološko raztopino, osušili in zaprli s polnilom Cavit. Drugi kočnik na obeh straneh nam je služil za kontrolo. Živali smo žrtvovali 5 minut, 6 ur, 24 ur in 72 ur po posegu ter na histoloških rezinah pod svetlobnim in na ultratankih rezinah pod elektronskim mikroskopom kvalitativno in polkvantitativno analizirali odziv zobne pulpe. Rezultati: Pod jedkano kaviteto je bila 5 minut po posegu odontoblastna plast bolj neurejena, 6 in 24 ur po posegu je bilo v njej vidnih manj odontoblastov in hiperemija v pulpi je bila obsežnejša kakor pod nejedkano kaviteto. Pod jedkano kaviteto je bilo 72 ur po posegu tudi manj znakov obnove, vnetni odziv v pulpi pa je bil po obeh posegih enak. Z ultrastrukturno analizo smo prikazali apoptozo, kot možno obliko celične smrti po obeh posegih. Zaključek: Jedkanje kavitete dodatno poškoduje odontoblaste. Sočasno z dentinogenezo se v pulpi pojavi vnetje, zato bi bilo potrebno raziskavo dopolniti z analizo prisotnosti bakterij v kaviteti.
Descriptors		DENTAL PULP ACID ETCHING, DENTAL DENTAL PULP CAVITY ODONTOBLASTS RATS, WISTAR DENTAL CAVITY PREPARATION