Author/Editor		Szepfalusi, Z
Title		Izvor zgodnje alergijske senzibilizacije
Translated title		Origin of early sensitisation to allergens
Type		članek
Source		In: Gregorič A, editor. Nutritivna alergija. Farmakologija perinatalnega obdobja. Skrb za zdravje v prvem letu življenja. Zbornik predavanj 13. srečanje pediatrov v Mariboru z mednarodno udeležbo; 2003 apr 4-5; Maribor. Maribor: Splošna bolnišnica Maribor,
Publication year		2003
Volume		str. 28-32
Language		eng
Abstract		The pre- and postnatal environment appears to be of crucial importance for the manifestation of allergic diseases which often begins during infancy. Although T cell reactivity of foetal origin to a range of common allergens is present in most cord blood samples, the immunological basis remains unclear. In order to test the hypothesis of transplacental allergen transfer we studied double-sided open ex vivo perfusion experiments of isolated placental cotyledons with the nutritive allergens beta-lactoglobulin (BLG) and ovalbumin (OVA) and the inhalant major birch pollen allergen Bet v1. Full term and preterm placentas were obtained immediatelyafterdelivery to recover functionally active maternal and foetal circulations. Thus, a foetal arteryand a foetal vein were cannulated and perfused with pure medium (foetoplacental circulation), whereas the intervillous space of placentas was flushed with allergen containing medium by puncture of the basal plate (maternoplacental circulation). Samples which were collected throughout perfusion experiment from foetal venous outflow were tested by allergen-specific enzymelinked immunosorbent assays (ELISA) for the presence of allergens indicative of materno-foetal transplacental passage. We observed transplacental transfer of BLG, OVA, and Bet v1 in placentas of term as well as premature deliveries. The respective allergen was readily detectable in foetal effluent at the beginning of the perfusion experiment and allergen levels reached a plateau after about two hours. The steady state transfer rate of BLG and OVA in term placentas was 0.012 % ± 0.001% and 0.013 % ± 0.001% of total dose, i.e. 130.21 ± 7.41 ng/ml and 115.83 ± 6.07 ng/mL, respectively. (Abstract truncated at 2000 characters).
Descriptors		IMMUNIZATION MATERNAL-FETAL EXCHANGE ALLERGENS FOOD HYPERSENSITIVITY INFANT OVALBUMIN LACTOBACILLUS POLLEN