| Author/Editor | Blomgren, Bo; Bohm-Starke, Nina; Falconer, Christian; Hilliges, Marita | |
| Title | A computerised stereological method for quantitative estimation of surface area of blood vessels | |
| Type | članek | |
| Source | Image Anal Stereol | |
| Vol. and No. | Letnik 20, št. Suppl 1 | |
| Publication year | 2001 | |
| Volume | str. 129-32 | |
| Language | eng | |
| Abstract | A computerised method for contextual detection and stereological measurement of immunostained blood vessels in the vestibulum vulvae is discussed. The aim of the study was to investigate if the erythema seen in the gynaecological disorder vestibulitis can be explained by increase or growth of the vessels in the tissue, by using a newly developed computer-assisted stereological measurement program. Biopsies were taken from vestibulum vulvae of 10 healthy women and 10 women with vestibulitis, fixated and immunostained with antibodies against factor VIII to reveal the endothelial cell lining in the blood vessels. Thereafter images were captured from the sections, enhanced and processed with the stereological measurement program to generate a list of intercept points for the final calculation of vessel area fraction per tissue volume (mm2/mm3). The measurement results showed no statistically significant difference between patients with vestibulitis compared to the control group. The computer program, however, was found easy to learn and use, and the overall procedure was found stable and error-free. With this computer-assisted stereological measurement program, the investigator is able to view and work with the images directly on the computer screen, instead of having to make photocopies and put on measurement grids. The measurement procedure is made quicker, easier and more accurate and unbiased, as the computer performs the measurements and calculations. However, the procedure still relies on some interaction from the investigator, in compensating for errors due to irregularities in tissue preparation. | |
| Descriptors | VULVITIS ENDOTHELIUM, VASCULAR IMAGE PROCESSING, COMPUTER-ASSISTED IMMUNOHISTOCHEMISTRY BIOPSY |