| Author/Editor | Volavšek, Metka | |
| Title | Spremembe genov in proteinov celičnega ciklusa pri ploščatoceličnem karcinomu grla in spodnjega žrela | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Medicinska fakulteta | |
| Publication year | 2003 | |
| Volume | str. 92 | |
| Language | slo | |
| Abstract | Squamous cell carcinoma of the head and neck region (HNSCC) is a group of cancers with similar histology but to some extent, different etiology and biological behaviour, relating mainly to the site of origin. Despite improvements in diagnosis and new modalities of treatment, the survival rate has remained relatively low over the last two decades. A better understanding of molecular mechanisms involved in the development and progression of HNSCC is needed to achieve more successfull treatment of the patients. The major mechanisms involved in genomic instability during tumor progression are loss of heterozygosity (LOH) and microsatellite instability (MSI). The most frequently affected are the tumor suppressor genes (TSG). The significance of these molecular genetic changes in the pathogenesis of HNSCC is not yet completely elucidated. In order to determine the role, nature and possible prognostic significance of the most frequent genetic changes in the development of this cancer, immunohistochemical and molecular genetic analysis was performed on 101 squamous cell carcinomas of larynx and hypopharynx (LHSCC). Since disregulation of the cell cycle is one of major characteristics of malignant tumors, we preferentially examined the participant TSG p53, p21, p16, Rb, and oncogene cyclin D1. Additionally, chromosomal region harbouring potential TSG FHIT was examined. Surgical specimens of all patients with LHSCC treated during the period from 1996 to June 1999 were included in the study. Formalin fixed and paraffin embedded tumor specimens were graded according to the criteria of the WHO classification into three grades using light microscopy. In specimens where different grades of epithelial hyperplastic lesions (EHL) were found adjacent to the cancer, they were graded according to the Ljubljana classification. (Abstract truncated at 2000 characters). | |
| Descriptors | LARYNGEAL NEOPLASMS HYPOPHARYNGEAL NEOPLASMS CARCINOMA, SQUAMOUS CELL CELL CYCLE PROTEINS RETROSPECTIVE STUDIES IMMUNOHISTOCHEMISTRY PROTEIN P53 GENES, P53 CYCLINS HETEROZYGOTE DETECTION MICROSATELLITE REPEATS ELECTROPHORESIS, POLYACRYLAMIDE GEL DNA FRAGMENTATION BASE SEQUENCE POLYMERASE CHAIN REACTION SURVIVAL ANALYSIS |