Author/Editor		Bervar, Aleš
Title		Lokalizacija katepsinov B in L ter njunih inhibitorjev, stefinov A in B, v procesu invazije pri celičnih linijah tumorja dojke
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		2003
Volume		str. 125
Language		slo
Abstract		Clinicians use prognostic factors to determine the course of therapy in breast cancer patients. Classic prognostic factors, such as tumor size, histology, infiltration into lymph nodes, etc., are no longer sufficient for adequate patient classification into risk groups. That can lead to inadequate use of adjuvant therapy after surgical resection of the breast. In the search for new prognostic factors, methods that allow insight to the conditions within a single cell as well as molecular methods are often being used. It is difficult to obtain appropriate tumor samples for this kind of research, therefore models of cell lines with differing in vivo tumorigenicities are frequently used. In this thesis, we used an established cell line model comprising four breast cancer cell lines. MCFlOA was the parental noninvasive cell line, the second was its ras-transfectant MCFlOA-NeoT with low in vivo tumorigenicity. The other two lines, MCFIOAT-Cala and MCFlOAT-Cald, were prepared from tumors in mice inoculated with the MCF l0A-NeoT line. In our work, we concentrated on the role of cathepsins and their inhibitors in cancer development, specifically in cell invasion through the extracellular matrix and metastasis formation. Cathepsins are involved in the proteolytic cascade, in which they activate other proteinases (plasmin, metalloproteinases) and actively degrade components of the extracellular matrix. This process enables cells to penetrate the surrounding tissue and form secondary tumors. Increases in cathepsin activity and concentration have been linked to worse prognosis in several studies. Therefore, we postulated that their concentration should be related to in vivo tumorigenicity. (Abstract truncated at 2000 characters).
Descriptors		BREAST NEOPLASMS CATHEPSINS CATHEPSIN B CYSTEINE PROTEINASE INHIBITORS NEOPLASM INVASIVENESS NEOPLASM METASTASIS TUMOR CELLS, CULTURED MICROSCOPY, CONFOCAL IMAGE PROCESSING, COMPUTER-ASSISTED