Author/Editor		Potokar, M; Milisav, I; Kreft, M; Stenovec, M; Zorec, R
Title		Apoptosis triggered redistribution of caspase-9 from cytoplasm to mitochondria
Type		članek
Source		Febs Lett
Vol. and No.		Letnik 544, št. 1-3
Publication year		2003
Volume		str. 153-9
Language		eng
Abstract		Caspase-9 is an apoptosis initiator protease activated as a response to the mitochondrial damage in the cytoplasmic complex apoptosome. By fluorescence labelling of proteins, confocal microscopy and subcellular fractionations we demonstrate that caspase-9 is in the cytoplasm of non-apoptotic pituitary cells. The activation of apoptosis with rotenone triggers the redistribution of caspase-9 to mitochondria. Experiments using the general caspase inhibitor z-VAD.fmk and the specific caspase-9 inhibitor z-LEHD.fmk show that the caspase-9 redistribution is a regulated process and requires the activity of a caspase other than the caspase-9. We propose that this spatial regulation is required to control the activity of caspase-9.
Descriptors		PITUITARY GLAND APOPTOSIS TRANSFECTION CYSTEINE PROTEINASES MICROSCOPY, CONFOCAL IMMUNOHISTOCHEMISTRY POLYMERASE CHAIN REACTION