Author/Editor		Potočnik, Uroš; Glavač, Damjan; Ravnik-Glavač, Metka
Title		Identification of novel genes with somatic frameshift mutations within coding mononucleotide repeats in colorectal tumors with high microsatellite instability
Type		članek
Source		Genes Chromosomes Cancer
Vol. and No.		Letnik 36
Publication year		2003
Volume		str. 48-56
Language		eng
Abstract		We have systematically retrieved genes with coding mononucleotide repeats from sequence databases and analyzed them for mutations in tumors with high levels of microsatellite instability (MSI-H). We found somatic frameshift mutations in 7/ I 3 genes previously not analyzed in MSI-H tumors. According to the frequency of mutations in MSI-H tumors, these genes could be divided into genes with high coding mononucleotide repeat instability (CMRI-H) and genes with low coding mononucleotide instability (CMRI-L). CMR-H genes were mutated in more than 9/38 and CMRI-L in less than 4/38 of MSI-H tumors. Four genes in our study were CMRI-H and could thus possibly play a role in the development of MSI-H tumors: TFE3 (9/38), TEF4 ( I 2/38), RGS 12 ( I I /38), and TCFI ( I 2/38). Our results suggest that systematic identification of genes with CMR in the sequence databases and determination of mutation frequency in MSI-H tumors might be a powerful tool for identification of new molecular targets in the development of MSI-H tumors.
Descriptors		FRAMESHIFT MUTATION MICROSATELLITE REPEATS NUCLEOTIDES COLORECTAL NEOPLASMS ADENOCARCINOMA SEX FACTORS DATABASES, FACTUAL INTESTINAL MUCOSA