Author/Editor		Mueller-Premru, Manica; Beović, Bojana
Title		Odvzem in transport kužnin in detekcija mikroorganizmov v hemokulturi
Type		članek
Source		In: Baklan Z, Reberšek-Gorišek J, Kotnik-Kervokljan B, editors. Zbornik predavanj in praktikum Bedjaničev simpozij z mednarodno udeležbo o temi Sepsa in septični šok; 2003 maj 31-31; Maribor. Maribor: Splošna bolnišnica Maribor,
Publication year		2003
Volume		str. 37-47
Language		slo
Abstract		The microbiological diagnosis of sepsis is necessary fo reveal the etiologic agent and to treat the patient properly. The quality of all the steps in this process, from obtaining blood for cultures, to transport and adequate detection of bacteriemia, are important: In this study we wanted to emphasize the importance of following the blood culture guidelines and to show the blood culture isolates and their antimicrobial susceptibility. 81ood should be obtained aseptically after skin disinfection from the peripheral vein during the rise of body temperature, prior to the administration of systemic antimicrobial fherapy, and directly inoculated into fhe blood culture media. It is obvious to take 2-3 blood cultures, containing enough blood (5 - 10 ml per bottle). The transport to the laboratory should be as quick as possible. In the year 2001 at the Institute of Microbiology and Immunology 1 : 300 blood cultures from 5.079 patients in the University Clinical Centre and at the Institute of Oncology were analyzed. They were incubated in the continuous-monitoring blood culture system. When positive, blood cultures were Gram stained and reported to the department. Bacteria were identified and antimicrobial susceptibility was determined according to NCCLS sfandards. Blood cultures were positive in 647 (12,7%) patients, who had 1040 positive blood cultures. In 86 (13,3%) patients bacteriemia was polymicrobial. The most frequent isolates were Escherichia coli in 134 (20,7%) patients and Staphylococcus aureus in 109 (16,9%) patients. Coagulase-negative staphylococci were isolated in 142 (21,9%) patients, but were commonly regarded as contaminants. Susceptibility of E. coli to ampicillin was 50,8 I and to ciprofloxacin 86,6%. Methicillin-susceptibility in S. aureus was 85,3%. The majority of patients with bacteriemia were from the Department of Infectious diseases and other Departmenfs of internal medicine.
Descriptors		SEPSIS BLOOD SPECIMEN COLLECTION BACTEREMIA ESCHERICHIA COLI STAPHYLOCOCCUS AUREUS STREPTOCOCCUS PNEUMONIAE PSEUDOMONAS AERUGINOSA DRUG RESISTANCE, MICROBIAL