Author/Editor		Kranjc, Simona; Čemažar, Maja; Grošel, Alenka; Pipan, Živa; Serša, Gregor
Title		Effect of electroporation on radiosensitization with cisplatin in two cell lines with different chemo- and radiosensitivity
Translated title		Učinek elektroporacije s cisplatinom na radiosenzibilizacijo dveh celičnih linij z različno občutljivostjo za kemoterapevtike in obsevanje
Type		članek
Source		Radiol Oncol
Vol. and No.		Letnik 37, št. 2
Publication year		2003
Volume		str. 101-7
Language		eng
Abstract		Aim. Radiosensitization with cisplatin can be enhanced by electroporation of cells and tumours. The aim of this study was to extend our previous studies on two carcinoma tumour models with different chemo-and radiosensitivity in order to evaluate whether this treatment is effective also on less chemo-and radiosensitive tumour cells. Materials and methods. This in vitro study was performed on carcinoma SCK and EAT-E cells. The cytotoxicity of three-modality treatment consisting of cisplatin, electroporation and irradiation was determined by the clonogenic assay. Results. The radiosensitizing effect of cisplatin on the two cell lines was greatly enhanced by electroporation. By this combined treatment, less chemo and radiosensitive EAT-E cells were rendered as sensitive as more chemo and radiosensitive SCK cells. Conclusion. The enhancement of cisplatin-induced radiosensitization of cells by electroporation could be beneficially used in the treatment of intrinsically less chemo- and radiosensitive tumours.
Summary		Izhodišča. Z elektroporacijo celic in tumorjev lahko povečamo njihovo občutljivost za cisplatin. Namen raziskave je bil razširiti našo predhodno raziskavo na dva tumorska modela karcinoma z različno občutljivostjo za kemoterapijo in obsevanje, ter ugotoviti, ali je takšno zdravljenje učinkovito tudi pri celicah, ki so manj občutljive za kemoterapijo in obsevanje. Materiali in metode. V in vitro raziskavi smo uporabili celični liniji karcinoma SCK in EAT-E. Citotoksično delovanje kombiniranega zdravljenja s cisplatinom, elektroporacijo in obsevanjem smo določili s testom klonogenosti. Rezultati. Z elektroporacijo se je radiosenzibilizirajoči učinek cisplatina na testiranih celičnih linijah zelo povečal. Manj kemo- in radio-občutljive celice EAT-E so po kombiniranem zdravljenju postale enako občutljive kot celice SCK, ki so bolj občutljive za kemoterapijo in obsevanje. Zaključki. Povečano občutljivost celic za cisplatin po elektroporaciji lahko koristno uporabimo tudi za zdravljenje manj občutljivih celic za kemoterapijo in obsevanje.
Descriptors		TUMOR CELLS, CULTURED ELECTROPORATION DRUG DELIVERY SYSTEMS CISPLATIN RADIATION TOLERANCE CELL SURVIVAL