| Author/Editor | Bizjak-Mali, Lilijana | |
| Title | Vpliv stradanja na ultrastrukturo hepatocitov močerila (Proteus anguinus, Amphibia, Urodela) | |
| Translated title | Influence of the starvation on the ultrastructure of the hepatocytes in Proteus anguinus (Amphibia, Urodela) | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Biotehniška fakulteta, Oddelek za biologijo | |
| Publication year | 2003 | |
| Volume | str. 116 | |
| Language | slo | |
| Abstract | Proteus anguinus, the European endemic cave salamander is well adapted to the cave environment. Reduced metabolic rate, starvation resistance and energy rich substances in the liver focused our attention and led our investigation on the morphology of its liver during food deprivation. Histological, histochemical and ultrastructural features of the liver of P. anguinus were investigated with light and electron microscopy, after food deprivation periods of one, four and eighteen months, respectively. The results were compared with fed individuals and field animals. Glycogen and proteins concentrations in the liver were determined spectrofotometrically. Total fats were estimated by Folch method. With the method of quantitative analysis of lipid droplets in hepatocytes we would like to confirm the hypothesis that the lipids are utilized during food deprivations. The present study indicates that during starvation hepatocytes undergo ultrastructural changes related to synthesis, storage and release of reserve material. The morphological changes of hepatocytes during starvation are mainly concerned with distribution of the cytoplasmic organelles and reserve materials in the hepatocytes. After one month of food deprivation the differences in distribution and appearance of glycogen have been observed. Glycogen was present in monoparticulate forms (`beta-particles) and was more dispersed in the cytoplasm without any clumps. But after eighteen months of fasting glycogen was abundant, mainly in monoparticulate forms and formed intense clumos throughout the cytoplasm. The PAS positive reaction was intense. The lipid droplets, which were numerous and with intense fuzzy coat of glycogen in one month starved animals, they were slightly diminished after eighteen months of fasting. (Abstract truncated at 2000 characters). | |
| Descriptors | STARVATION LIVER GLYCOGEN PROTEINS LIPIDS PROTEIDAE MICROSCOPY, ELECTRON HISTOCYTOCHEMISTRY PERIODIC ACID-SCHIFF REACTION SPECTROPHOTOMETRY MITOCHONDRIA, LIVER MICROBODIES GOLGI APPARATUS |