Author/Editor | Božič, Mojca; Stegnar, Mojca | |
Title | Validation of an automated immunoturbidimetric assay for measurement of plasma D-dimer | |
Type | članek | |
Source | Clin Chem Lab Med | |
Vol. and No. | Letnik 41, št. 7 | |
Publication year | 2003 | |
Volume | str. 958-62 | |
Language | eng | |
Abstract | The performance characteristics and diagnostic accuracy of a new rapid automated quantitative immunoturbidimetric D-dimer assay, Auto-Dimer (Biopool, Umea, Sweden) were evaluated in a population of 135 outpatients with suspected deep-vein thrombosis of a lower limb. Enzyme-linked immunosorbent assay was used as the reference method. The Auto-Dimer assay showed good reproducibility. The correlation between Auto-Dimer and enzyme-linked immunosorbent assay was high (r = 0.91, p < 0.05) and agreement in classifying patients above or below the cut-off was good IK coefficient 0.74, 95% CI 0.62-0.86). At a cut-off of 340 micro g/I the sensitivity and specificity of Auto-Dimer were high (100% and 61%, respectively). These data show that Auto-Dimer is a reliable screening test for exclusion of deep-vein thrombosis. The assay could be included in prospective patient management studies in order to obtain further information on its clinical usefulness. | |
Descriptors | DIMERIZATION VEINS THROMBOSIS NEPHELOMETRY AND TURBIDIMETRY ANTIBODIES, MONOCLONAL CALIBRATION SENSITIVITY AND SPECIFICITY ENZYME-LINKED IMMUNOSORBENT ASSAY |